Product nameTransferrin Human SimpleStep ELISA? Kit See all Transferrin kitsDetection methodColorimetricPrecision Inter-assay Sample n Mean SD CV% Overall 3 4.2% Sample typeCell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate PlasmaAssay typeSandwich (quantitative)Sensitivity 30 pg/mlRange30 pg/ml - 20000 pg/mlRecovery Sample specific recovery Sample type Average % Range Serum 103 101% - 106% Cell culture media 88 87% - 89% Heparin Plasma 104 101% - 107% EDTA Plasma 103 * - 104% Citrate Plasma 101 * - 102% Assay time1h 30m Assay durationOne step assaySpecies reactivityReacts with: Human Does not react with Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, PigProduct overview Abcam’s Transferrin in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin protein in human serum, plasma and cell culture supernatant samples. The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. Notes Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Tested applicationsSandwich ELISAmore details PlatformMicroplate Properties Storage instructionsStore at +4°C. Please refer to protocols. Components 1 x 96 tests 10X Transferrin Capture Antibody 2 x 300μl 10X Transferrin Detector Antibody 2 x 300μl 10X Wash Buffer PT 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 2 x 3ml Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml Transferrin Human Lyophilized Purified Protein 2 vials