Product nameCorticosterone ELISA kitDetection methodColorimetricPrecision Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.1% Tests1 x 96 well plateSample typeCell culture supernatant, Saliva, Milk, Urine, Serum, PlasmaAssay typeCompetitiveSensitivity= 0.3 ng/mlRange0.391 ng/ml - 100 ng/mlRecovery = 96 % Assay time3h 00m Assay durationMultiple steps standard assaySpecies reactivityReacts with: Mouse, Rat, Human, Baboon Product overview Abcam’s Corticosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant. A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate. Tested applicationsCompetitive ELISAmore details PlatformMicroplate