Oxford Expression Technologies 重組蛋白
Oxford Expression Technologies公司簡(jiǎn)介
Oxford Expression Technologies（英國(guó)）位于英國(guó)牛津，為全球制藥和生物技術(shù)行業(yè)提供產(chǎn)品，服務(wù)和咨詢服務(wù)。
Oxford Expression Technologies 簡(jiǎn)稱（OET）專門從事重組蛋白表達(dá)，其核心技flashBACTM是一種基于桿狀病毒的蛋白表達(dá)系統(tǒng)，能快速生產(chǎn)適用于小型和大型蛋白表達(dá)系統(tǒng)的多種重組病毒。
Oxford Expression Technologies簡(jiǎn)稱（OET）產(chǎn)品還涉及昆蟲和哺乳動(dòng)物表達(dá)系統(tǒng)中的載體、轉(zhuǎn)染試劑和培養(yǎng)基。
蛋白質(zhì)印跡
該分析使用凝膠電泳分離的提取物中抗體與變性蛋白質(zhì)的結(jié)合。它對(duì)于識(shí)別與其他蛋白質(zhì)的脫靶結(jié)合非常有用。它還可以與二維分析相結(jié)合，二維分析在第一維通過電荷分離蛋白質(zhì)，然后在第二維通過大小分離。這種更先進(jìn)的方法可識(shí)別與預(yù)期蛋白質(zhì)大小相同的非靶蛋白是否與抗體結(jié)合。
共聚焦顯微鏡
我們可以使用牛津布魯克斯大學(xué)（Oxford Brookes University）全guo最xian進(jìn)的共聚焦顯微鏡設(shè)備之一。共聚焦顯微鏡是研究抗體在細(xì)胞內(nèi)非變性狀態(tài)下與其靶標(biāo)相互作用的理想選擇。任何所需的細(xì)胞培養(yǎng)物都可以用重組桿狀病毒或陰性對(duì)照感染（昆蟲）或轉(zhuǎn)導(dǎo)（哺乳動(dòng)物和禽）。然后用一抗處理細(xì)胞，然后用標(biāo)記的二抗孵育，該二抗在適當(dāng)波長(zhǎng)的光下產(chǎn)生熒光。
酶聯(lián)免疫吸附法
這是抗體最常見的應(yīng)用之一，特別是在診斷試劑盒中。至關(guān)重要的是，使用的任何抗體都可以被信任以結(jié)合正確的蛋白質(zhì)靶標(biāo)。在此類測(cè)試中，經(jīng)過驗(yàn)證的陽性對(duì)照至關(guān)重要。雖然可以使用來自表達(dá)重組蛋白的細(xì)胞的粗提取物，但最好使用純化的材料。這也是提供陰性對(duì)照很重要的地方。我們可以模擬從這些樣品中提取蛋白質(zhì)，并證明重組蛋白來源中沒有其他任何東西可以與靶抗體結(jié)合。
宿主細(xì)胞的選擇
大多數(shù)重組蛋白表達(dá)系統(tǒng)局限于狹窄范圍的宿主細(xì)胞。桿狀病毒系統(tǒng)的一個(gè)主要優(yōu)點(diǎn)是，它可用于在生產(chǎn)性病毒感染后在昆蟲細(xì)胞中制造蛋白質(zhì)，或通過轉(zhuǎn)導(dǎo)在哺乳動(dòng)物和禽類細(xì)胞培養(yǎng)物中制造蛋白質(zhì)。由于蛋白質(zhì)的翻譯后修飾可能是抗體結(jié)合的關(guān)鍵因素，因此使用來自細(xì)胞的材料來測(cè)試這一點(diǎn)是值得的，因?yàn)檫@些材料與預(yù)期靶標(biāo)密切相關(guān)。
Oxford Expression Technologies公司的熱銷產(chǎn)品：
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
flashBAC | OET-100150 | 5 reactions |
flashBAC | OET-100151 | 24 reactions |
flashBAC | OET-100152 | 96 reactions |
flashBAC GOLD | OET-100200 | 3 reactions |
flashBAC GOLD | OET-100201 | 5 reactions |
flashBAC GOLD | OET-100202 | 24 reactions |
flashBAC GOLD | OET-100203 | 96 reactions |
flashBAC ULTRA | OET-100300 | 5 reactions |
flashBAC ULTRA | OET-100301 | 24 reactions |
flashBAC ULTRA | OET-100302 | 96 reactions |
flashBAC ULTRA | OET-100304 | 3 reactions |
flashBAC selection box 1 | OET-100400 | 3 x 3 reactions |
flashBAC selection box 2 | OET-100401 | 4 x 3 reactions |
flashBAC PRIME | OET-100500 | 5 reactions |
flashBAC PRIME | OET-100501 | 24 reactions |
baculoQUANT All-in-one virus extraction & titration kit | OET-100602 | 100 reactions |
titrePLUS flashBAC all-in-one | OET-100710 | 5 + 100 reactions |
titrePLUS flashBAC all-in-one | OET-100711 | 24 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100712 | 5 + 100 reactions |
titrePLUS flashBAC GOLD all-in-one | OET-100713 | 24 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100714 | 5 + 100 reactions |
titrePLUS flashBAC ULTRA all-in-one | OET-100715 | 24 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100716 | 5 + 100 reactions |
titrePLUS flashBAC PRIME all-in-one | OET-100717 | 24 + 100 reactions |
BacPAK6 Linearised DNA | OET-101101 | 5 reactions |
BacPAK6 Linearised DNA | OET-101102 | 24 reactions |
BacPAK6 Linearised DNA | OET-101103 | 96 reactions |
BacPAK6 sec+ Linearised DNA | OET-101104 | 5 reactions |
BacPAK6 sec+ Linearised DNA | OET-101105 | 24 reactions |
BacPAK6 sec+ Linearised DNA | OET-101106 | 96 reaction kit |
pOET sequencing primers | OET-200100 | 2 x 100 ul |
pOET1 transfer plasmid | OET-200101 | 10 ug |
pOET1N_6xHis transfer plasmid | OET-2001011 | 10 ug |
pOET1C_6xHis transfer plasmid | OET-2001012 | 10 ug |
pOET2 transfer plasmid | OET-200103 | 10 ug |
pOET2N/C_6xHis transfer plasmid | OET-2001031 | 10 ug |
pOET2C_6xHis transfer plasmid | OET-2001032 | 10 ug |
pOET3 transfer plasmid | OET-200104 | 10 ug |
pOET4 transfer plasmid | OET-200105 | 10 ug |
pOET5 transfer plasmid | OET-200106 | 10 ug |
pOET6 BacMAM transfer plasmid | OET-200107 | 10 ug |
pOET1 Gateway® transfer plasmid | OET-200108 | 6 ug |
pOET6 BacMAM Gateway® transfer plasmid | OET-200109 | 6 ug |
pOET8.VE1 transfer plasmid | OET-200121 | 10 ug |
pOET8.VE2 transfer plasmid | OET-200122 | 10 ug |
pOET8.VE3 transfer plasmid | OET-200123 | 10 ug |
baculoFECTIN II | OET-300105 | 150 ul |
baculoFECTIN II | OET-300106 | 1ml |
baculoCOMPLETE protein expression kit | OET-400100 | 5 reactions |
baculoCOMPLETE protein expression kit + baculoQUANT all-in-one | OET-400101 | 5 + 100 reactions |
