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登革熱NS1檢測(cè)卡
產(chǎn)品規(guī)格:25T/盒;
保存條件:避光 4-30度 保存。
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登革熱NS1檢測(cè)卡
我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲(chóng)病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。
以下是我司出售的部分登革熱產(chǎn)品
登革熱NS1抗原快速檢測(cè)卡 |
登革熱NS1 ELISA檢測(cè)試劑盒 |
登革熱IgG/IgM快速檢測(cè)試劑盒 |
登革熱IgG快速檢測(cè)試劑盒 |
登革熱IgM快速檢測(cè)試劑盒 |
登革熱IgG ELISA檢測(cè)試劑盒 |
登革熱IgM ELISA檢測(cè)試劑盒 |
登革熱IgM捕獲法ELISA檢測(cè)試劑盒 |
登革熱IgG間接法ELISA檢測(cè)試劑盒 |
登革熱NS1檢測(cè)卡
15. Read the absorbance of each well at 450 nm having blanked the plate reader against a chromogen blank composed of 100 µl each of Stabilized Chromogen and Stop Solution. Read the plate within 2 hours after adding the Stop Solution.
16. Plot the absorbance of the standards against the standard concentration. (Optimally, the background absorbance may be subtracted from all data points, including standards, unknowns and controls, prior to plotting.) Draw the best smooth curve through these points to construct the standard curve. If using curve fitting software, the four parameter algorithm provides the best curve fit.
17. Read the protein concentrations for unknown samples and controls from the standard curve plotted in step 16. Multiply value(s) obtained for sample(s) by the appropriate dilution factor to correct for the dilution with Standard Diluent Buffer. (Samples producing signals higher than the highest standard should be further diluted in Standard Diluent Buffer and reanalyzed, multiplying the concentration by the appropriate dilution factor.)
十。喺450 nm處有沖板嘅讀者對(duì)由100個(gè)µl個(gè)個(gè)穩(wěn)定發(fā)色團(tuán)與閘住液空白讀各井嘅吸光度。喺加閘住液后2鐘內(nèi)讀板。
16。根據(jù)標(biāo)準(zhǔn)濃度繪制標(biāo)準(zhǔn)吸光度。(Optimally,喺繪制之前,可以喺所有資料啲減去背景吸光度,包標(biāo)準(zhǔn)、未知數(shù)同控制)。通過(guò)呢啲啲繪制*平滑曲線(xiàn)嚟構(gòu)造標(biāo)準(zhǔn)曲線(xiàn)。如果使用曲線(xiàn)擬合軟件,四引數(shù)算灋講嚇嘢喇!*曲線(xiàn)擬合。
17。由第16步中繪制嘅標(biāo)準(zhǔn)曲線(xiàn)讀取未知樣品嘅蛋白質(zhì)濃度同控制。用適當(dāng)嘅稀釋因子獲得樣品嘅倍增值,用標(biāo)準(zhǔn)Diluent Buffer較稀釋值。(樣品生產(chǎn)信號(hào)高于zui高標(biāo)準(zhǔn)應(yīng)進(jìn)一步稀釋嘅標(biāo)準(zhǔn)Diluent Buffer同再分析,再乘以適當(dāng)嘅稀釋濃度嘅因素。)