鼠抗人CD74單克隆抗體

廣州健侖生物科技有限公司

CD74表達(dá)于所有的B細(xì)胞和某些T細(xì)胞亞類（MHC II 陽(yáng)性），主要是生發(fā)中心的淋巴細(xì)胞，具有幾種異構(gòu)體（33-41KDa），是HLA-DR的恒定鏈。此抗體主要用于研究B細(xì)胞及其來(lái)源的腫瘤，T細(xì)胞淋巴瘤很少有表達(dá)，染色模式為細(xì)胞膜，但可見(jiàn)核旁顆粒狀染色，有助于淋巴瘤和白血病的研究。此外，可用于研究非典型纖維黃色肉瘤和惡性纖維組織細(xì)胞瘤。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿/細(xì)胞膜

克隆號(hào)：LN2

同型：IgG1

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

Percll是一種無(wú)毒，惰性，且與生物膜不發(fā)生粘附的物質(zhì)。用它制成的梯度可在室溫下放置數(shù)星期而梯度的形狀不發(fā)生絲毫變化。Percoll密度梯度離心已被許多學(xué)者用來(lái)分離各種動(dòng)物的抗原抗體，而用于分離早期生精細(xì)胞的報(bào)道不多。Bucci等［8］用組合酶消化，Percoll等密度梯度離心分離了大鼠的A型精原細(xì)胞，所獲細(xì)胞純度達(dá)到51%。Hofmann等［5］用Percoll不連續(xù)密度梯度離心，從10d小鼠睪丸分離生殖細(xì)胞，結(jié)果精原細(xì)胞主要分布于密度為1.030的Pecoll梯度中。但文中沒(méi)有報(bào)告所獲細(xì)胞的純度。
本實(shí)驗(yàn)結(jié)果表明，11%～19%Percoll梯度之間主要為死細(xì)胞及細(xì)胞碎片19%～27%及35%～43%Percoll梯度之間主要是大量支持細(xì)胞和少量管周肌樣細(xì)胞。精原細(xì)胞主要分布于27%～35%Percoll梯度間。電鏡觀察表明，該帶中大部分細(xì)胞的形態(tài)結(jié)構(gòu)特征與相同日齡的小鼠睪丸切片上精原細(xì)胞的形態(tài)結(jié)構(gòu)*。該帶細(xì)胞接種后，根據(jù)精原細(xì)胞與睪丸體細(xì)胞貼壁速度快慢的差異，利用選擇性貼壁法，待睪丸體細(xì)胞開(kāi)始貼壁極化而精原細(xì)胞仍然懸浮時(shí)(體外培養(yǎng)約3～4h)，將其進(jìn)一步純化后另行培養(yǎng)；待其貼壁后，依據(jù)其形態(tài)學(xué)特征進(jìn)行計(jì)數(shù)，結(jié)果精原細(xì)胞的純度平均達(dá)到68.76%，高于Bucci等的分離效果。本實(shí)驗(yàn)中，支持細(xì)胞在3個(gè)梯度中都有大量分布，可能與幼年鼠支持細(xì)胞非常豐富且其大小很不*有關(guān)。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲(chóng)病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

Percll is a non-toxic, inert substance that does not adhere to biofilms. Gradients made with it can be left at room temperature for several weeks without a gradual change in the shape of the gradient. Percoll density gradient centrifugation has been used by many researchers to isolate antigen-antibody of various animals, and few reports have been made on the isolation of early spermatogenic cells. Bucci et al [8] digested by combinatorial enzymes, Percoll density gradient centrifugation of rat spermatogonia type A, the purity of cells obtained 51%. Hofmann et al [5] used Percoll discontinuous density gradient centrifugation to separate germ cells from the 10-day mouse testis. As a result, spermatogonia were mainly distributed in a Pecoli gradient with a density of 1.030. However, the purity of the cells obtained is not reported in the text.
The results of this experiment show that between 11% and 19% of the Percoll gradients are predominantly a large number of supporting cells and few peritubular myoidoid cells between 19% to 27% of dead cells and cell debris and 35% to 43% of the Percoll gradient. Spermatogonia mainly distributed in 27% ~ 35% Percoll gradient. Electron microscopy showed that the morphology of most cells in the band was consistent with that of spermatogonia on the testicular sections of mice of the same age. After the cells are inoculated, the spermatogonia begin to attach to the parietal polarization and the spermatogonium still remain in suspension according to the difference of the adherent rate between spermatogonia and testicular somatic cells (in vitro culture for about 3 ~ 4h). After further purification, the cells were cultured separay. After being adhered, the purity of spermatogonia was 68.76% on average, which was higher than that of Bucci. In this experiment, the supporting cells in a large number of three gradient distribution, may be very rich in juvenile mouse supporting cells and its size is inconsistent.
First, the principle
Living cells contain LDH in their cytoplasm. Under normal circumstances, LDH can not penetrate the cell membrane, when the cells are NK cell-killing, LDH release to the extracellular. LDH dehydrogenates lithium lactate, which in turn reduces NAD to NADH, which in turn reduces Iodonitetrazolyltetrazolium (INT) via the dehydro-phenazine dimethyl ester sulfate (PMS), which undergoes H + reduction Magenta A month like compounds. 490nm colorimetric assay on a microplate reader.
Second, equipment and materials
A microplate reader, a microplate reader, a microplate reader, a YP-1 cell, a Hank's solution (pH 7.2 to 7.4), a complete RPMI1640 medium, lithium lactate or sodium lactate, nitrotetrazolium chloride (INT) (PMS), NAD, 0.2 mol / L Tris-HCl buffer (pH 8.2), 1% NP40 or 2.5% Triton