CD138肝素硫酸酯蛋白聚糖

廣州健侖生物科技有限公司

CD138（Syndecan-1)是跨膜硫酸乙酸肝素蛋白聚糖家族的成員，通過(guò)其硫酸乙酸肝素鏈共價(jià)結(jié)合多種胞外配體。CD138 是一種細(xì)胞粘附分子，具有促進(jìn)細(xì)胞增殖，細(xì)胞與基質(zhì)、細(xì)胞與細(xì)胞之間粘附等多種功能。CD138 在某些腫瘤的表達(dá)明顯缺失，導(dǎo)致細(xì)胞喪失生長(zhǎng)接觸抑制的功能，使得瘤細(xì)胞大量增殖，并具*的侵襲活性。CD138表達(dá)于正常淋巴細(xì)胞不同的分化階段，如前 B細(xì)胞、未成熟 B 細(xì)胞和漿細(xì)胞，同時(shí)還表達(dá)于上皮細(xì)胞、胚胎間質(zhì)細(xì)胞、血管平滑肌細(xì)胞、內(nèi)皮細(xì)胞和神經(jīng)細(xì)胞的基底面。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲(chóng)病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：B-A38

同型：IgG1

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD138肝素硫酸酯蛋白聚糖

實(shí)驗(yàn)原理
有些細(xì)菌具有合成淀粉酶的能力，可以分泌胞外淀粉酶。淀粉酶可以使淀粉水解為麥芽糖和葡萄糖，淀粉水解后遇碘不再變藍(lán)色。
細(xì)菌產(chǎn)生的脂肪酶能分解培養(yǎng)基中的脂肪生成甘油及脂肪酸。脂肪酸可以使培養(yǎng)基pH下降，可通過(guò)在油脂培養(yǎng)基中加入中性紅做指示劑進(jìn)行測(cè)試。中性紅指示范圍為pH6.8（紅）～8.0（黃）。當(dāng)細(xì)菌分解脂肪產(chǎn)生脂肪酸時(shí)，則菌落周?chē)囵B(yǎng)基中出現(xiàn)紅色斑點(diǎn)。
某些細(xì)菌分泌蛋白酶分解明膠，產(chǎn)生小分子物質(zhì)。如果細(xì)菌具有分解明膠的能力，則培養(yǎng)基可由原來(lái)固體狀態(tài)變成液體狀態(tài)。
牛乳中主要含有乳糖、酪蛋白等成分。細(xì)菌對(duì)牛乳的利用主要是指對(duì)乳糖及酪蛋白的分解和利用。牛乳中常加入石蕊作為酸堿指示劑和氧化還原指示劑。石蕊中性時(shí)呈淡紫色，酸性時(shí)呈紅色，堿性時(shí)呈藍(lán)色，還原時(shí)則部分或全部脫色。細(xì)菌對(duì)牛乳的利用可分三種情況：
（1）酸凝固作用：細(xì)菌發(fā)酵乳糖后，產(chǎn)生許多酸，使石蕊牛乳變紅，當(dāng)酸度很高時(shí)，可使牛乳凝固，此稱(chēng)為酸凝固。
（2）凝乳酶凝固作用：某些細(xì)菌能分泌凝乳酶，使牛乳中的酪蛋白凝固，這種凝固在中性環(huán)境中發(fā)生。通常這種細(xì)菌還具有水解蛋白質(zhì)的能力，因而產(chǎn)生氨等堿性物質(zhì)，使石蕊變藍(lán)。
（3）胨化作用：酪蛋白被水解，使牛乳變成清亮透明的液體。胨化作用可以在酸性條件下或堿性條件下進(jìn)行，一般石蕊色素被還原褪色。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲(chóng)病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱(chēng)】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

Experimental principle
Some bacteria have the ability to synthesize amylase, which can secrete extracellular amylase. Amylase can hydrolyze starch to maltose and glucose, and iodine no longer turns blue after hydrolysis of starch.
Bacteria produce lipase can decompose the fat in the medium to produce glycerol and fatty acids. Fatty acids reduce the pH of the medium and can be tested by adding neutral red as an indicator in fat medium. Neutral red indicates a range of pH6.8 (red) to 8.0 (yellow). When bacteria break down fat to produce fatty acids, red spots appear in the medium around the colonies.
Some bacteria secrete protease to decompose gelatin to produce small molecules. If bacteria have the ability to break down gelatin, the medium can change from the original solid state to a liquid state.
Milk mainly contains lactose, casein and other ingredients. The use of bacteria by milk mainly refers to the decomposition and utilization of lactose and casein. Milk often add litmus as an acid-base indicator and redox indicator. Litmus lavender when neutral, acidic red, alkaline when blue, restore some or all of the decolorization. Bacteria on the utilization of milk can be divided into three situations:
(1) acid coagulation: Bacteria ferment lactose, resulting in many acids, the litmus milk turns red, when the acidity is high, the milk can coagulate, this is called acid coagulation.
(2) Rennet coagulation: Some bacteria can secrete rennet to coagulate casein in milk, which occurs in a neutral environment. Often this bacterium also has the ability to hydrolyze proteins, thus producing alkaline substances such as ammonia, turning the litmus bluish.
(3) peptonization: casein is hydrolyzed, so that the milk into a clear and transparent liquid. Peptonization can be carried out under acidic conditions or under alkaline conditions, and the litmus pigment is generally reduced and discolored.