pET-32a（+）

D0064     pET-32a（+）    NobleRyder 25ug（0.5OD） 560.00

載體類型：大腸桿菌表達(dá)載體
載體大?。?/span> 5900 bp
載體宿主：大腸桿菌（E.coli）
細(xì)菌抗性：氨芐青霉素（Amp）
5' 測(cè)序引物：S Tag primer
3' 測(cè)序引物：T7 terminator primer
載體簡(jiǎn)介：
The pET-32 series is designed for cloning and high-level expression of peptide sequences fusedwith the 109aa Trx Tag thioredoxin protein . Cloning sites are available for producing fusionproteins also containing cleavable His Tag and S Tag sequences for detection and puri cation.Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression regionof the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented
so that infection with helper phage will produce virions containing single-stranded DNA that corre-sponds to the coding strand. Therefore, single-stranded sequencing should be performed using theT7 terminator primer.