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CYT-308 | 重組人巨噬細胞集落刺激因子 (rHuM-CSF) | 2μg/10μg/1mg | Prospec |
Background: | |
Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs).A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo | |
![]() | Description : |
Recombinant Human M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD Recombinant M-CSF is purified by proprietary chromatographic techniques. | |
![]() | Physical Appearance: |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
![]() | Formulation: |
The lyophilized protein (1mg/ml) was lyophilized with 0.1gr HSA and 0.6gr Manntiol. | |
![]() | Solubility: |
It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
![]() | Stability: |
Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution M-CSF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
![]() | Purity: |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
![]() | Amino acid sequence: |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser. | |
![]() | Dimers and aggregates: |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
![]() | Biological Activity: |
ProSpec’s M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 105 IU/mg. | |
![]() | Endotoxin: |
Less than 0.1 ng/µg (IEU/µg) of M-CSF. | |
![]() | Protein content: |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of M-CSF as a Reference Standard. | |
![]() | Usage: |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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