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重組人肌肉生長抑素（rHuMyostatin）返回列表頁

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詳情介紹

CYT-418

重組人肌肉生長抑素（rHuMyostatin）

2ug/10ug

Prospec

Description:
	Myostatin （GDF8, MSTN） is a member of the transforming growth factor beta superfamily that is essential for proper regulation of skeletal muscle mass.Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. The myostatin pathway is conserved and regulates muscle mass in a number of animal species ranging from fish to humans. Inhibition of myostatin using a variety of therapeutic approaches can increase muscle mass in a number of animal models of human disease, including muscular dystrophy.
	Description:
	Recombinant Human Myostatin also called GDF-8 produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 109 amino acids and having a total molecular mass of 24814 Dalton. Recombinant Human Myostatin is purified by proprietary chromatographic techniques
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Lyophilized from a concentrated （1mg/ml） solution containing no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized Myostatin in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized Recombinant Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Myostatin should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s Recombinant Human Myostatin is fully biologically active when compared to standard. The ED50 as determined by the inhibition of the prolifiration of MPC-11 cells is < 20 ng/ml.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Recombinant Human Myostatin.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.