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Roche11585614910 DIG-High Prime DNA Labeling and Detection Starter

參考價(jià) ￥ 4998
訂貨量 ≥1
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廣州市齊云生物技術(shù)有限公司位于廣州市越秀區(qū)，是一家專業(yè)從事科研用生化試劑及相關(guān)產(chǎn)品銷售的公司。公司產(chǎn)品涉及生化試劑、標(biāo)準(zhǔn)品、ELISA試劑盒、RT-PCR相關(guān)試劑、基因檢測試劑、農(nóng)業(yè)殘留檢測試劑等眾多領(lǐng)域，致力于為生物企業(yè)、科研院所、醫(yī)療機(jī)構(gòu)提供*的生化試劑、生物產(chǎn)品服務(wù)。


         公司自成立以來，與國內(nèi)外企業(yè)，科研院校和多家代理商建立了良好的合作關(guān)系，是國內(nèi)產(chǎn)品系列多，品種全，質(zhì)量優(yōu)的生物產(chǎn)品和生化試劑供應(yīng)商。能夠滿足各生物企業(yè)、科研院所、醫(yī)療機(jī)構(gòu)的各種需求，能*提供一站式試劑供應(yīng)服務(wù)。專業(yè)企業(yè)，專業(yè)品質(zhì)，專業(yè)服務(wù)，“廣州齊云，試劑專家”，公司立志于做廣州地區(qū)*的科研試劑經(jīng)銷商


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生化試劑,標(biāo)準(zhǔn)品,ELISA試劑盒,RT-PCR相關(guān)試劑,基因檢測試劑,農(nóng)業(yè)殘留檢測試劑等

DIG-High Prime DNA Labeling and Detection Starter Kit II

Kit for chemiluminescent detection with CSPD, ready-to use.

Cat.No. Pack Size Qty Price Options
11585614910 1 kit for 12 labeling reactions and detection of 24 blots 10 ng to 3 μg per assay, blots of 100 cm2 4998

 

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence （probe concentrations at 20 ng/ml）. A single copy human gene （t-PA） is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

  1. DIG-High Prime, 5x conc., 50 µl
  2. DIG-labeled Control DNA, 20 µl, （5 µg/ml） pBR328 （linearized with Bam HI）
  3. DNA Dilution Buffer, 3 x 1 ml
  4. Anti-digoxigenin-AP Conjugate, 50 µl
  5. CSPD, ready-to-use, 50 ml
  6. Blocking Solution, 10x conc., 4 x 100 ml
  7. DIG Easy Hyb Granules, 4 x 100 ml


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