Roche11585606910 DIG-High Prime
| 參考價(jià) | ￥ 6997.2 |
| 訂貨量 | ≥1 |
- 公司名稱 廣州市齊云生物技術(shù)有限公司
- 品牌
- 型號(hào) Roche11585606910
- 產(chǎn)地 美國羅氏
- 廠商性質(zhì) 代理商
- 更新時(shí)間 2017/9/28 2:13:59
- 訪問次數(shù) 645
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廣州市齊云生物技術(shù)有限公司位于廣州市越秀區(qū)，是一家專業(yè)從事科研用生化試劑及相關(guān)產(chǎn)品銷售的公司。公司產(chǎn)品涉及生化試劑、標(biāo)準(zhǔn)品、ELISA試劑盒、RT-PCR相關(guān)試劑、基因檢測(cè)試劑、農(nóng)業(yè)殘留檢測(cè)試劑等眾多領(lǐng)域，致力于為生物企業(yè)、科研院所、醫(yī)療機(jī)構(gòu)提供*的生化試劑、生物產(chǎn)品服務(wù)。
公司自成立以來，與國內(nèi)外企業(yè)，科研院校和多家代理商建立了良好的合作關(guān)系，是國內(nèi)產(chǎn)品系列多，品種全，質(zhì)量優(yōu)的生物產(chǎn)品和生化試劑供應(yīng)商。能夠滿足各生物企業(yè)、科研院所、醫(yī)療機(jī)構(gòu)的各種需求，能*提供一站式試劑供應(yīng)服務(wù)。專業(yè)企業(yè)，專業(yè)品質(zhì)，專業(yè)服務(wù)，“廣州齊云，試劑專家”，公司立志于做廣州地區(qū)*的科研試劑經(jīng)銷商
主要代理品牌：
生化試劑,標(biāo)準(zhǔn)品,ELISA試劑盒,RT-PCR相關(guān)試劑,基因檢測(cè)試劑,農(nóng)業(yè)殘留檢測(cè)試劑等
DIG-High Prime |
| For the nonradioactive labeling of DNA with DIG-11-dUTP alkali-labile, using random oligonucleotides as primers. |
| Cat.No. | Pack Size | Qty | Price | Options |
|---|---|---|---|---|
| 11585606910 | 160 µl for 40 labeling assays 0.01-3 µg template DNA per assay | 6997.2 | ||
Application
DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
The standard reaction with 1 µg template DNA will routinely give a yield of 0.8 µg DIG-labeled DNA after 1 hour, and 2.3 µg after a 20-hour incubation.
labeled DNA probes are used in Southern, northern, and dot blots, as well as in colony or plaque hybridization.
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1 µg total human DNA.
The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond （Figure 1）, enables easier and more efficient stripping and reprobing of blots.
Benefits
- DNA amounts ranging from 10 ng to 3 µg in a standard reaction.
- DNA of different lengths ranging from small restriction fragments to λ or cosmid DNA.
- DNA, supercoiled or linearized.
- DNA in low melting-point agarose.
Product Description
A standard labeling reaction with 1 µg template yields 0.8 µg newly synthesized digoxigenin-labeled DNA after 1 hour, and 2 µg after a 20-hour incubation at 37°C.
Background Information
, a novel 5x conc. labeling mixture contains optimal concentrations of random primers, nucleotides, and DIG-dUTP, alkali-labile, in a highly efficient reaction buffer with Klenow enzyme. Simply add to the denatured template DNA. The number of pipetting steps are thus reduced to a minimum, while reproducibility and accuracy of experiments are effectively increased.
DIG-labeled probes in the reaction mix or in the hybridization buffer are stable for more than 12 months stored at -15 to -25°C. They can be reused several times if freshly denatured before use.
Figure 1: Structure of alkali-labile DIG-11-dUTP.
Contents
Typical Experiment
Table 1: Yield of labeling reaction.
Quality
In a standard assay with 1 µg linearized pBR 328, 0.8 µg of DIG-labeled DNA is synthesized after 1 hour, and 2.3 µg after 20 hours. When this labeled DNA is used for hybridization at a concentration of 20 ng/ml, 0.03 pg homologous DNA are detected by chemiluminescence with the anti-DIG-alkaline phosphatase conjugate and CSPD on a dot or Southern blot.
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