—、多色流式分析的福音

由于熒光光譜的重疊現象，在進行流式多色分析時，必須設置單染管進行熒光補償。OneComp /UltraComp eBeads 微球大小和淋巴細胞相當，可連接各種熒光標記的抗體來調節(jié)補償。

每滴微球中都含有兩群：一群是可以結合抗體的陽性群，一群是不會與抗體反應的陰性群。

優(yōu)點：

★ 使用方便，一滴即可。
★ 與所有小鼠、大鼠和倉鼠來源的抗體反應，要求抗體來源是Kappa鏈。
★ 適用于各種激發(fā)光---適用于488 nm 藍光，532 nm 綠光，561 nm 黃光，633-635 nm 紅光，UltraComp eBeads尤其適用紫外 (355 nm) 或者 紫色 (405 nm) 激光器。

Comparison of BD CompBead and BD CompBead Plus particles on different sample types

A. H9 human embryonic stem cells (WiCell, Madison, WI), and BD CompBead (Cat. No. 552843) and BD CompBead Plus particles (Cat. No. 560497) were stained with PE mouse anti–SSEA-4 (Cat. No. 560128) and analyzed on a flow cytometer. Both unstained and stained hESCs are shown in blue, negative and stained BD CompBead particles in green, and negative and stained BD CompBead Plus particles in red. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

B. Lysed whole blood (LWB), BD CompBead particles, and BD CompBead Plus particles were stained with PE mouse anti-human CD3 (Cat. No. 555333) and analyzed on a flow cytometer. Stained LWB is shown in blue, negative and stained BD CompBead particles in green, and negative and stained BD CompBead Plus particles in red. Flow cytometry was performed on a BD LSR II flow cytometry system

產品訂購信息：

1、CompBead Compensation Particles通用補償微球

2、CompBead Plus Particles 補償大微球，適用于larger cell types, for example, human embryonic stem cells (hESCs)