BTX特殊電極的應(yīng)用

此次只將貼壁轉(zhuǎn)染、活體等進行總結(jié)，對于大規(guī)模的轉(zhuǎn)基因的應(yīng)用，由于BTX公司有新的發(fā)展，故此次未將其列入，待下次完善后補充。

1、         Adherent Cell Transfections (ACT)貼壁細胞轉(zhuǎn)染

•        In-situ electroporation of cells in the dish or on a slide.

  在培養(yǎng)皿或玻片上細胞的原位電穿孔   

•        Applicable to primary cells (HUVEC, primary Neurons, etc.) and other adherent cell lines.

 用于原代細胞（人臍靜脈內(nèi)皮細胞，原代神經(jīng)細胞等）和其他貼壁細胞系

Benefits of ACT 貼壁細胞轉(zhuǎn)染優(yōu)點

•          Increases cell viability 增加細胞活性

•          Decreases cell loss減少細胞損失

•          Increases efficiency增加效率

•          Minimizes experimental error將實驗失敗降至zui低

2、         In-Vivo Gene Delivery (IVGD)活體轉(zhuǎn)基因

Delivery of DNA directly into tissues of live animals using electroporation.  Facilitates studies in gene therapy, DNA vaccination, cancer therapy,  transdermal drug/gene delivery, etc.

使用電穿孔方法直接將DNA轉(zhuǎn)入活的動物組織，有利于基因治療、DNA疫苗、腫瘤治療等的研究

Benefits of IVGD活體轉(zhuǎn)基因的優(yōu)點

•        High level and long lasting expression

         高水平長效表達

•        No toxicity (compared to lipofection)

         無毒（相對于脂質(zhì)法）

•        No risk of viral infection (compared to viral delivery methods)

         無病毒感染的風(fēng)險（相對于病毒法）

•        Minimum tissue damage (compared to biolistics)

         zui小的組織傷害（相對于基因槍法） 

•        Ease of use

          容易使用

•         Applicable to various animal types such as mice, rats, rabbits, dogs, cats, pigs and monkeys

        可用于不同類型的動物  如：小鼠、大鼠、兔子、狗、貓、豬、和猴子

3、         In Ovo Gene Delivery 卵的轉(zhuǎn)基因

Electroporation in ovo gene delivery requires the use of genetrodes to deliver electrical pulses into developing chick embryos. The avian system is classically used to investigate questions of vertebrate development. Information gained on chick embryogenesis and fate of cells over time after genetic alteration is very valuable.

電穿孔用于卵的轉(zhuǎn)基因需要使用genetrodes來向發(fā)育中的雞胚傳遞電脈沖。這個鳥的系統(tǒng)是用來研究脊椎動物的經(jīng)典模型。在雞胚發(fā)生和轉(zhuǎn)基因發(fā)生后的結(jié)果是非常有價值的

Benefits of IOGD

•  High efficiency

•   No toxicity無毒

•   Ease of use易用

•   Localized expression局部表達

4、          New Applications新的應(yīng)用

•  In Vitro Embryo Gene Delivery (IVEGD) or In Utero Embryo Gene Delivery (IUEGD) - i.e. Mouse embryos

  體外胚胎轉(zhuǎn)基因或者子宮胚胎轉(zhuǎn)基因—如老鼠的胚胎

•  Organotypic culture electroporation (ie. Brain slices)

  器官培養(yǎng)電穿孔（如腦片）

•  Intact Plant Gene Delivery (IPGD) : using Tweezertrode, Caliper Electrodes or 2 Needle Arrays to deliver molecules into intact plant tissues. No need to digest cell wall. Efficient method for transgenic plant generation.

  完整植物轉(zhuǎn)基因：使用Tweezertrode, Caliper Electrodes or 2 Needle Arrays 電極將分子轉(zhuǎn)入完整植物組織。無需將細胞壁消化。是產(chǎn)生轉(zhuǎn)基因植物的有效方法