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Western blot should be performed under non-reducing conditions.
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
PLoS genetics
Genetic mechanism of human neutrophil antigen 2 deficiency and expression variations.
"MA1-19250 was used in flow cytometry to study the genetic mechanism responsible for expression variations and deficiency in human neutrophil antigen 2"
Authors Li Y,Mair DC,Schuller RM,Li L,Wu J
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