Complex of recombinant human mTOR (mechanistic target of rapamycin kinase), recombinant human MLST8 (mTOR associated protein, LST8 homolog), and recombinant human Raptor (regulatory associated protein of mTOR complex 1). The mTOR construct contains an N-terminal Flag-Tag encompassing amino acids 1362-2549(end). The MLST8 construct contains an N-terminal His-tag (6xHis), full length, encompassing amino acids 2-326(end). The Raptor construct contains an N-terminal His-tag (6xHis), full length encompassing amino acids 2-1335(end). Complex was co-expressed and affinity purified.

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