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Fura-2 AM

CAS No. : 108964-32-5

產(chǎn)品活性：Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ indicator. Storage: protect from light.

研究領域：Others

作用靶點：Fluorescent Dye

In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1.First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2.Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3.Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4.Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5.Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6.Mount the coverslip on the imaging chamber.

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