The Neutrophil Elastase Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of elastase for screening and profiling applications. The elastase assay kit comes in a convenient 96-well or 384-well format and contains enough purified elastase, fluorochrome-conjugated substrate, and PR-02 buffer for 100 or 400 reactions. This kit also includes Sivelestat as control inhibitor.

Figure 1: Illustration of the assay principle.
PR Substrate 3 is a fluorogenic peptide substrate for neutrophil elastase. In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Proteolysis releases AMC and fluorescence is emitted. The increase in fluorescence is directly proportional to elastase activity.