The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligａnd binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

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