不同規(guī)格電擊管電壓換算方法

時(shí)間：2024/5/7閱讀：267

不同電擊管的電壓參數(shù)需要換算，時(shí)間相同。以20ul電擊管參數(shù)為基準(zhǔn)，100ul比20ul約上調(diào)10V；200ul是20ul的約2.4倍；1000ul是20ul約2.5倍；120ul的是20ul的約1.4倍，600ul的是20ul的約1.6倍。下面是轉(zhuǎn)換示例：

20ul：500V

100ul: 510V （20ul電壓+10V）

200ul：1200V （20ul電壓的2.4倍）

1000ul：1250V （20ul電壓的2.5倍）

120ul：700V （20ul電壓的1.4倍）

600ul：800V （20ul電壓的1.6倍）

*部分單核細(xì)胞相關(guān)的細(xì)胞系，電轉(zhuǎn)質(zhì)粒后幾乎所有電轉(zhuǎn)成功的細(xì)胞會(huì)死亡，而電轉(zhuǎn)mRNA，siRNA和Cas9 RNP沒關(guān)系。Celetrix公司專門開發(fā)了試劑盒可以幫助單核細(xì)胞類的細(xì)胞系進(jìn)行質(zhì)粒電轉(zhuǎn)，目前驗(yàn)證過THP-1和U937這兩種細(xì)胞系電轉(zhuǎn)效果非常好，而Molm-13和Sup-M2細(xì)胞電轉(zhuǎn)質(zhì)粒細(xì)胞死亡問題還沒有解決。

Cell Type	Mode	20ul, 100ul	200ul, 1ml
Human PBMC	PBMC	1200 -1400	NA
Human T cells, unstimulated	PBMC	1300 -1450	NA
Human B cells, unstimulated	PBMC	1300 -1450	NA
Human NK cells, unstimulated	PBMC	1300 -1450	NA
Human Neutrophil	PBMC	1300 -1450	NA
Mouse lymph node primary T cell	PBMC	1300 -1450	NA
Mouse primary spleen cells	PBMC	1300 -1450	NA
Mouse bone marrow monocyte	PBMC	1300 -1450	NA

Human Cultured T cells	Cell Line	480-540	1150-1300
Human Cultured NK cells	Cell Line	480-540	1200-1300
Human iPSC	Cell Line	500-540	1200-1300
Human MM1.S	Cell Line	520-560	1250-1350
Human adipose MSC	Cell Line	500-540	1200-1300
Human bone marrow MSC	Cell Line	500-540	1200-1300
Human cord blood MSC	Cell Line	500-540	1200-1300
Human ESC derived neural stem cell	Cell Line	520-560	1250-1350
Human primary hepatocyte	Cell Line	480-520	1150-1250
Human primary Dendritic cells	Cell Line	480-520	1150-1200
Human neural progenitor ReNCell VM	Cell Line	520-560	1250-1350
Mouse iPSC	Cell Line	480-520	1150-1250
Mouse ESC	Cell Line	480-520	1150-1250
Mouse lymph node Cultured T cell	Cell Line	500-540	1200-1300
Mouse peritoneal Macrophage	Cell Line	540-580	1350-1400
Primary mouse cortical neurons	Cell Line	520-560	1350-1400
Rat primary cortex neural progenitor cell	Cell Line	520-560	1250-1350

293	Cell Line	440-480	1050-1150
293T	Cell Line	460-500	1050-1150
A20	Cell Line	540-560	1250-1350
A549	Cell Line	540-560	1250-1350
ADSCs	Cell Line	500-520	1200-1300
ARPE-19	Cell Line	520-540	1250-1350
BaF3	Cell Line	520-540	1250-1350
BJ	Cell Line	500-520	1250-1400
CAL51	Cell Line	500-520	1250-1350
CHO-K1	Cell Line	560-600	1250-1400
CHO-S	Cell Line	520-580	1250-1400
DEL	Cell Line	500-540	1200-1300
DLD1	Cell Line	500-520	1200-1300
EMT6	Cell Line	500-520	1200-1300
HCT116	Cell Line	540-560	1200-1300
Hela	Cell Line	480-520	1250-1350
Hepa1-6	Cell Line	500-520	1200-1300
Hep3b	Cell Line	500-540	1100-1200
HSF	Cell Line	520-540	1250-1350
HUC-Maaa13-P3	Cell Line	520-540	1250-1350
HUVEC	Cell Line	520-560	1250-1350
H1975	Cell Line	520-560	1250-1350
H22	Cell Line	500-520	1200-1300
H226	Cell Line	520-540	1250-1350
H9C2	Cell Line	500-540	1350-1400
HL-60 *	Cell Line	520-560	1250-1350
iMC	Cell Line	500-540	1200-1300
Jurkat	Cell Line	500-540	1200-1350
Karpas299	Cell Line	520-560	1250-1350
K562	Cell Line	480-520	1200-1300
MC38	Cell Line	500-520	1200-1300
MCF-7	Cell Line	500-520	1200-1300
MDA-MB-231	Cell Line	500-520	1220-1300
Molm-13 *	Cell Line	500-540	1250-1350
Nalm-6	Cell Line	520-540	1250-1350
NK-92	Cell Line	440-460	1150-1200
Raji	Cell Line	520-540	1250-1350
RAW264.7	Cell Line	520-540	1250-1350
SF9 insect cell	Cell Line	480-520	1200-1300
SK-HEP-1	Cell Line	480-520	1220-1300
SP2/0	Cell Line	480-500	1150-1200
SUP-M2 *	Cell Line	520-560	1250-1350
Swiss-3T3	Cell Line	520-540	1250-1350
p3X63Ag8.653	Cell Line	500-540	1220-1300
THP-1 *	Cell Line	500-540	1250-1300
U251	Cell Line	480-520	1250-1400
U937 *	Cell Line	500-540	1250-1300

*Many monocyte or macrophage related cell lines such as THP-1, U937, HL-60 and Molm13 will die out within two to three days after plasmid electroporation. These cells have no problem with mRNA, siRNA and Cas9 RNP electroporation. The efficiency would be nearly 100% and cell survival is not affected. Celetrix has developed the THP-1 Helper Kit for plasmid electroporation of monocytic cell lines. A significant portion of THP-1 and U937 cells can survive and grow with the helper kit, enabling easy generation of stable cell lines. Our THP-1 Helper Kit does not help cell lines such as Molm13 and SUP-M2 with plasmid electroporation and we are actively researching the solutions.

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