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TAMRA phosphoramidite, 5-isomer介紹
閱讀：201 發(fā)布時(shí)間：2023-1-13中文名：四甲基羅丹明-亞磷酰胺，5-異構(gòu)體
英文名：TAMRA phosphoramidite, 5-isomer
分子式：C40H50N5O6P
分子量：727.83
性狀：紫色固體
純度：≥95%
溶解度：good in DMF, DMSO
保存條件：避光，干燥，-20℃保存
注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。
用途：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的，非藥用，非食用
結(jié)構(gòu)式：
This phosphoramidite is used for synthesis of oligonucleotides 5’-labeled with TAMRA.
TAMRA (carboxytetramethylrhodamine) is a xanthene dye from the rhodamine family with emission in the orange spectrum range (maximum at 563 nm). This fluorophore is traditionally used as a FRET-acceptor (and a quencher) in a pair with fluorescein (FAM) due to significant overlapping of their spectra. Thus, this phosphoramidite is convenient for the synthesis of dual-labeled probes TaqMan, which contain 5’-terminal TAMRA and FAM in the middle of the sequence or at the 3’-end (using Fluorescein dT Phosphoramidite and FAM CPG, respectively).
TAMRA 5’-labeled oligonucleotides are commonly used for quantitative PCR and fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a detection channel for TAMRA frequently.
The TAMRA dye is not stable in the presence of ammonium and sterically non-hindered primary amines, so it is strongly recommended to follow specified conditions for labeled oligonucleotide deprotection.
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