DreamFect™ Transfection Reagent is based on an innovative biochemical method. It contains both a lipidic part and a cationic part allowing the association of nucleic acids with this transfection reagent and their delivery inside cells.

Due to its unique properties, DreamFect™ releases large DNA amounts in the cytosol increasing DNA nuclear delivery (TEE Technology). Consequently, DreamFect™ is highly efficient at low doses of nucleic acids. Therefore, less cationic lipids are used and the potential secondary effects are avoided.

Exceptional efficiency High reproducibility

Broad range of cell types

For all nucleic acids applications

Non toxic Serum compatible

Simple, rapid and easy-to-use

DREAMFECT TRANSFECTION REAGENT:

Sizes:

500 µL: 125-250 transfections with 1 µg of DNA

1000 µL: 250-500 transfections with 1 µg of DNA

5 mL: 1250-2500 transfections with 1µg of DNA

艾美捷DREAMFECT 500 轉(zhuǎn)染試劑#OZB-DF40500基于一種創(chuàng)新的生化方法。它包含脂質(zhì)部分和陽(yáng)離子部分，允許核酸與該轉(zhuǎn)染試劑結(jié)合并在細(xì)胞內(nèi)遞送。

由于其獨(dú)-特的性質(zhì)，DreamFect™在細(xì)胞質(zhì)中釋放大量DNA，增加DNA核遞送（TEE技術(shù)）。因此，DreamFect™在低劑量的核酸下非常有效。因此，使用較少的陽(yáng)離子脂質(zhì)，避免潛在的二次效應(yīng)。

DREAMFECT 500 轉(zhuǎn)染試劑特征：

高效率 高重現(xiàn)性

多種細(xì)胞類型

適用于所有核酸應(yīng)用

無(wú)毒血清兼容

簡(jiǎn)單、快速且易于使用

DREAMFECT 500 轉(zhuǎn)染試劑規(guī)格：

500 μL：用 125 μg DNA 轉(zhuǎn)染 250-1 次

1000 μL：用 250 μg DNA 轉(zhuǎn)染 500-1 次

5 mL：用 1250μg DNA 轉(zhuǎn)染 2500-1 次

圖1：DreamFect 在各種細(xì)胞系中的轉(zhuǎn)染效率。在1孔板中用10.5 μg /孔的pEGFP質(zhì)粒DNA轉(zhuǎn)染0x524個(gè)細(xì)胞。轉(zhuǎn)染后24小時(shí)通過(guò)流式細(xì)胞術(shù)測(cè)量轉(zhuǎn)染細(xì)胞的百分比。

圖2：DreamFect 與其他轉(zhuǎn)染試劑的效率比較。在1孔板中每孔轉(zhuǎn)染10 μgpLuc質(zhì)粒DNA和5 μLDreamFect Gold試劑的細(xì)胞系（1x324細(xì)胞/孔）。其他轉(zhuǎn)染試劑根據(jù)制造商的說(shuō)明進(jìn)行檢測(cè)。