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Echelon新產(chǎn)品：外泌體純化和標記試劑盒
閱讀：1717 發(fā)布時間：2020-11-20
ExoClean and ExoTracker
K-4300a, K-4300b, K-4300c, K-4300d (20 tests)
Description: Simple, quick protocols for labeling (ExoTracker) and isolating (ExoClean) extracellular vesicles (EVs) from biological samples*
Echelon’s ExoClean – EV Purification Kit and ExoTracker kits為標記和純化外顯體和細胞外囊泡（EV）提供了一個簡單可靠的系統(tǒng)。（1）The ExoClean – EV Purification Kit (貨號：K-4300a )通過將沉淀試劑與微離心柱配對，取代了與基于超離心的純化相關的時間和設備成本，此外可以去除與其他沉淀技術(shù)相關的有害污染物。
（2）The ExoTracker kits (貨號：K-4300b -d) 使該系統(tǒng)的實用性更進一步，可以用膜或RNA特異性標簽標記EV。
產(chǎn)品列表
Catalog # | Description | Quantity |
ExoClean (K-4300a)
K-4301 | EV Precipitation Reagent | 30 mL |
K-SPIN | Microcentrifuge Columns | 20 columns |
ExoTracker - Membrane (K-4300b)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
ExoTracker - RNA (K-4300c)
K-4300a | ExoClean | 1 kit |
K-4303 | EV RNA Label | 20 µL |
ExoTracker - Total (K-4300d)
K-4300a | ExoClean | 1 kit |
K-4302 | EV Membrane Label | 150 µg |
K-4303 | EV RNA Label | 20 µL |
操作流程：
1：Collect EV sample
2：Centrifuge at 2000g for 10 min to remove any debris or cells.
3：Transfer supernatant and precipitate cleared sample with-K4301 for 30 minutes at room temperature with mixing
4：Centrifuge precipitated sample at 1500g for 30 minutes at room temperature.
5：Discard the supernatant fraction and resuspend the precipitated pellet in 1x PBS.
6：Apply the resuspended pellet to the equilibrated K-SPINcolumn and incubate for 5 minutes at room temperature.
7：Centrifuge the column at 500g for 30 seconds at room temperature.
8：Collect and examine the eluted, purified EVs.
注：Exosomes and extracellular vesicles (EVs)-胞外體和胞外小泡
發(fā)表文獻：
1. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. Biomed Res Int. 2018;2018:8545347.
2. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7(3):789-804.
3. Deregibus MC, Figliolini F, D’Antico S, Manzini PM, Pasquino C, De Lena M, et al. Charge-based precipitation of extracellular vesicles. Int J Mol Med. 2016;38(5):1359-66.
默瑞生物：
默瑞(上海)生物科技有限公司，作為專業(yè)的生命科學產(chǎn)品推廣與服務商，以“創(chuàng)新，專業(yè)，共贏”的理念，不斷強化服務體系，提升客戶體驗，以莫納生物產(chǎn)品為主線，結(jié)合國內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標準完整解決方案，幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新，加速轉(zhuǎn)化，創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品，提升社會福祉，實現(xiàn)多方共贏。
默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導入TwistDx,博日
Jackson,izon,Biontex,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質(zhì)品牌，逐步培育成為專業(yè)用戶的選擇。