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當(dāng)前位置：> 供求商機(jī)> Human ACE2 & TMPRSS2 lung reporter cells

[供應(yīng)]Human ACE2 & TMPRSS2 lung reporter cells

貨物所在地：北京北京市

更新時間：2024-03-29 09:07:02

有效期：2024年3月29日 -- 2025年3月30日

已獲點(diǎn)擊：31

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Cells will undergo genotypic changes resulting in reduced responsiveness over time in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably genetic

Contents and Storage

• 3-7 x 106 A549-ASCoV2 cells in a cryovial or shipping flask IMPORTANT: If cells provided in a cryovial are not frozen upon arrival,  contact InvivoGen immediately. 

• 1 ml of Blasticidin(10 mg/ml). Store at 4°C or at -20°C.* • 1 ml of Normocin™ (50 mg/ml), a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20°C.* 

*The expiry date is specified on the product label. Handling Frozen Cells Upon Arrival Cells must be thawed immediately upon receipt and grown according  to handling procedures (as described on the next page) to ensure the  best cell viability and proper assay performance. Note: Avoid freezing cells upon receipt as it may result in irreversible  damage to the cell line. Disclaimer: We cannot guarantee cell viability if the cells are not thawed  immediately upon receipt and grown according to handling procedures. 

IMPORTANT: For cells that arrive in a shipping flask please refer to the  enclosed ‘cell recovery procedure’. 

Cell Line Stability 

Cells will undergo genotypic changes resulting in reduced  responsiveness over time in normal cell culture conditions. Genetic  instability is a biological phenomenon that occurs in all stably genetically  engineered cells. Therefore, it is critical to prepare an adequate number  of frozen stocks at early passages. To ensure maximum efficiency, do not  passage A549-ASCoV2 cells more than 20 times. 

 Quality Control 

• The induction of ASC::GFP expression has been verified by western  blot and fluorescence microscopy. 

• The overexpression of the human ACE2 (hACE2) gene has been verified by flow cytometry and functional assays.

• The overexpression of the human TMPRSS2 (hTMPRSS2) gene has been verified by RT-qPCR and functional assays.

• The stability of this cell line for 20 passages following thawing has been verified. 

• A549-ASCoV2 cells are guaranteed mycoplasma-free


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