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[供應]ST2339—分散酶II

貨物所在地：北京北京市

更新時間：2024-03-11 13:34:43

有效期：2024年3月11日 -- 2025年3月12日

已獲點擊：37

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The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml.

基本信息(General Information)：

Name (Chinese)分散酶II
Name (English)Dispase II
SpecificationsReagent grade, ≥0.5units/mg, protease
Chemical Formula
Synonym (Chinese)蛋白酶 來源于多粘芽孢桿菌
Synonym (English)Protease from Bacillus polymyxa
Beilstein Registry No.-
EINECS Number255-914-4
MDL NumberMFCD02253017
UNSPSC Code12352204

產(chǎn)品描述(Description)：

Analysis NoteThe enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival
ApplicationThe enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso
Biochem/physiol ActionsDispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes.
Physical formlyophilized powder containing calcium acetate and milk sugar
Preparation NoteA 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.
Unit DefinitionOne unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

性質(zhì)(Properties)：

activity≥0.5 units/mg solid
solubility10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble
storage temp.2-8°C


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