**Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)，數(shù)量4，售完為止。

產(chǎn)品名稱(chēng)：Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)
產(chǎn)品貨號(hào)：MLB00C

• Assay Length

  4.5 hours

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

• Sensitivity

  4.8 pg/mL

• Assay Range

  12.5 - 800 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

• Specificity

  Natural and recombinant mouse IL-1 beta

• Cross-reactivity

  < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

• Interference

  No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accuray. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.

Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate (1 KT)

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Recovery

The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

• Long Name

  Interleukin 1 beta

• Entrez Gene IDs

  3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig);

• Alternate Names

  catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;

Related Research Areas

• Cancer Biomarkers
• Conventional/Classical Dendritic Cells
• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Angiogenesis
• IL-1 Family
• Infectious Diseases
• Inflammatory Mediators
• Isolation, Culture, and Identification of Natural Killer Cells
• LTi Cells
• Macrophage Activation Markers
• Multiple Sclerosis and Myelin Related Molecules
• Myeloid-derived Suppressor Cells (MDSC)
• NCR-ILC3
• Neuroinflammation
• NOD-like Receptors and the Inflammasome
• Plaets
• Regulatory T Cells (Tregs)
• T Cell Cytokine Signaling
• Th17 Cells
• Th9 Cells