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CTCF CUTANA CUT RUN Antibody抗體
HA Tag CUTANA CUT RUN Antibody抗體
BRD4 CUTANA CUT RUN Antibody抗體
Epicypher 13-0041,Histone H3K4me3 A
CAS | 請詢 | 純度 | 請詢 |
---|---|---|---|
分子量 | 請詢 | 分子式 | 請詢 |
供貨周期 | 兩周 | 規(guī)格 | 50 Preps |
貨號 | 14-0050 | 應用領域 | 生物產業(yè),綜合 |
主要用途 | 科研 |
EpiCypher DNA純化試劑盒
采用專為低洗脫體積(低至6µL)設計的錐形凈化柱,可回收>50bp的DNA片段,只需30分鐘即可完成純化流程,是各種CUT&RUN靶點,包括組蛋白PTM和轉錄因子的理想選擇。
圖例:
![]() | Figure 1: CUT&RUN DNA fragment size distribution analysis 500,000 K562 cells per sample were processed with the CUTANA™ ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EpiCypher 13-2014) antibodies. Isolated DNA was used to prepare paired-end Illumina® sequencing libraries, which were analyzed by Agilent TapeStation® , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA + sequencing adapters). |
產品描述:
Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 µL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.
產品訂購
產品名稱 | 貨號 | 規(guī)格 |
CUTANA™ DNA Purification Kit | 14-0050 | 50 Preps |
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