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上海橋星貿(mào)易有限公司
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上海橋星貿(mào)易有限公司
主營(yíng)產(chǎn)品： 進(jìn)口透析袋,密理博ECL發(fā)光液,B27無(wú)血清培養(yǎng)基,Gibco膠原酶 |
聯(lián)系電話
M1705 MMLV逆轉(zhuǎn)錄酶 Promega試劑?
規(guī)格：50000U
M1705 MMLV逆轉(zhuǎn)錄酶 Promega試劑? 產(chǎn)品描述：
其它組分：
M-MLV Reverse Transcriptase【子貨號(hào)：M170B，包裝：1 x 50,000u，，運(yùn)保溫度：–20°C】
M-MLV RT 5X Buffer【子貨號(hào)：M531A，包裝：2 x 1ml，，運(yùn)保溫度：–20°C】
描述：
說(shuō)明 |
| Moloney Murine Leukemia Virus Reverse Transcriptase(MMLV RT，M-MLV 逆轉(zhuǎn)錄酶) 是一種RNA 依賴的DNA 聚合酶，可用于長(zhǎng)mRNA (>5kb) 為模板的cDNA 合成。該酶是M-MLV 的pol 基因產(chǎn)物，由一個(gè)分子量為71kDa 的亞單位組成。M-MLV RT 的RNase H 活性比常用的鳥(niǎo)類成髓細(xì)胞性白血病病毒(Avian Myeloblastosis Virus，AMV) 逆轉(zhuǎn)錄酶弱。
|
特點(diǎn) |
| • 提供5× 反應(yīng)緩沖液：250mM Tris-HCl (pH 8.3，25℃ )，375mM KCl，15mM MgCl2，50mM DTT。 |
原廠資料：
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71kDa. The RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.
Features - Benefits
Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
May Be Heat-Inactivated: M-MLV RT is inactivated by heating at 70°C for 10 minutes.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/
Applications
First-strand cDNA synthesis.
Primer extension.
References
Roth, M.J. et al. (1985) J. Biol. Chem. 260, 9326–35.
注意事項(xiàng)：
| 儲(chǔ)存條件 | 儲(chǔ)存于-20℃。 |
? 相關(guān)產(chǎn)品列表：
| Promega 系列 | ||
| 序號(hào) | 貨號(hào) 名稱 | 規(guī)格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
