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[供應(yīng)]M7745 Pfu DNA Polymerase 500u Promega試劑
M7745 Pfu DNA Polymerase 500u Promega試劑
貨物所在地:
上海上海市
產(chǎn)地:
Promega進(jìn)口
更新時間:
2025-01-19 21:00:07
有效期:
2025年1月19日--2025年7月19日
已獲點(diǎn)擊:
459
【簡單介紹】M7745 Pfu DNA Polymerase 500u Promega試劑
規(guī)格:500u
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【詳細(xì)說明】

M7745 Pfu DNA Polymerase 500u Promega試劑

規(guī)格:500U

M7745 Pfu DNA Polymerase 500u Promega試劑 產(chǎn)品描述:

其它組分:

 

Pfu DNA Polymerase【子貨號:M774B,包裝:1 x 500u,,運(yùn)保溫度:-20℃】

Pfu 10X Reaction Buffer w/20mM MgSO4【子貨號:M776A,包裝:3 x 1.2ml,,運(yùn)保溫度:-20℃】

 

 

描述:

 

 

說明

 

Pfu DNA Polymerase 是一種從強(qiáng)烈熾熱球菌(Pyrococcus furiosus ) 中分離得到的熱穩(wěn)定性酶,分子量大約為90kDa。該酶在75℃時可進(jìn)行DNA 復(fù)制,在鎂離子存在的條件下可催化核苷酸沿5` → 3` 方向發(fā)生聚合反應(yīng),形成雙鏈DNA。 Pfu DNA 聚合酶也具有3` → 5` 外切酶( 校正) 活性。當(dāng)聚合反應(yīng)發(fā)生堿基錯配時,聚合酶的校正活性可將錯配的堿基切除。因而,推薦Pfu DNA 聚合酶用于高保真的PCR 反應(yīng)和引物的延伸反應(yīng)。Pfu DNA 聚合酶產(chǎn)生的PCR產(chǎn)物為平端。

Pfu DNA 聚合酶的10× 反應(yīng)緩沖液( 含有MgSO4):200mM Tris-HCl (pH 8.8,25℃ ), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 和1mg/ml 無核酸酶的BSA。

 

特點(diǎn)

 

. 高保真:在所有熱穩(wěn)定性聚合酶中Pfu DNA 聚合酶的錯誤幾率zui低。

. 完備::提供含有20mM MgSO4 的10× 反應(yīng)緩沖液。

. *:Promega 公司的PCR 系統(tǒng),酶和試劑被證明在PCR中能得到可靠高效的結(jié)果。

 

 

 

原廠資料:

 

 

Pfu DNA Polymerase is a thermostable enzyme of approximay 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, PfuDNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

Pfu DNA Polymerase 10X Reaction Buffer with MgSO4: 200mM Tris-HCl (pH 8.8 at 25°C), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton® X-100 and 1mg/ml nuclease-free BSA.

Features - Benefits

  • High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase.
  • Complete: Provided with 10X buffer containing 20mM MgSO4.
  • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not compley satisfied with any Promega PCR product, we will send a replacement or refund your account.

Guarantee

PCR Satisfaction Guarantee

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not compley satisfied with any Promega PCR product we will send a replacement or refund your account.That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.

Applications

Pfu DNA Polymerase is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.

For more information, see the Protocols & Applications Guide.

References

  1. Fiala, G. and Stetter, K.O. (1986) Arch. Microbiol. 145, 56.
  2. Lundberg, K.S. et al. (1991) Gene 108, 1–6.
  3. Flaman, J.M. et al. (1994) Nucl. Acids Res. 22, 3259–60.
  4. Cline, J. et al. (1996) Nucl. Acids Res. 24, 3546–51.
  5. Andre, P. et al. (1997) Genome Res. 7, 843–52.

 

 

相關(guān)產(chǎn)品列表:

Promega 系列
序號貨號  名稱 規(guī)格
1M1801  T4連接酶         100U 
2M1804      T4連接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         


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