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[供應(yīng)]N2511 Recombinant RNasin Promega試劑
N2511 Recombinant RNasin Promega試劑
貨物所在地:
上海上海市
產(chǎn)地:
Promega進(jìn)口
更新時(shí)間:
2025-01-19 21:00:07
有效期:
2025年1月19日--2025年7月19日
已獲點(diǎn)擊:
342
【簡(jiǎn)單介紹】N2511 Recombinant RNasin Promega試劑
規(guī)格:2500u
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【詳細(xì)說明】

N2511 Recombinant RNasin Promega試劑?

原裝*,質(zhì)量保證,現(xiàn)貨供應(yīng)

規(guī)格:2500u

N2511 Recombinant RNasin Promega試劑? 產(chǎn)品描述:

其它組分:

 

Recombinant RNasin® Ribonuclease Inhibitor【子貨號(hào):N251A,包裝:1 x 2,500u,,運(yùn)保溫度:–20°C】

 

 

描述:

 

 

說明

 

天然的和重組的RNasin® 核酸酶抑制劑具有廣譜的抑制RNase 活性的性質(zhì),包括抑制中性型(neutral type) 真核RNase。該抑制劑的分子量為50kDa,按1:1 的比例與RNase 非共價(jià)結(jié)合發(fā)揮抑制作用。RNasin® Ribonuclease Inhibitor 與RNase( 如RNase A) 結(jié)合的Ki 值大約是10-14M。Promega 提供兩種不同的抑制劑:天然的RNasin® Ribonuclease Inhibitor 和重組的RNasin® Ribonuclease Inhibitor。兩種產(chǎn)品均通過離子交換和親和層析相結(jié)合的純化方法純化得到。

 

特點(diǎn)

 

•  抑制常見的真核RNase:RNasin® Ribonuclease Inhibitor 具有廣譜RNase 抑制特性,包括 RNase A、 RNase B、 RNase C 和人類胎盤RNase,不抑制RNase T1、 S1 核酸酶、曲霉(Aspergillus ) 中的 RNase 以及RNase H、RNase ONE® Ribonuclease。

兼容性:RNasin® Ribonuclease Inhibitor 不抑制SP6、T7 或T3RNA Polymerase、ImProm-II™、AMV 或M-MLV Reverse Transcriptase 或Taq DNA Polymerase。

pH 范圍寬:在較寬的pH 范圍內(nèi)(pH 5-8) 均可發(fā)揮作用。

 

 

 

原廠資料:

 

RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www.promega。。com/custom/

 

Features - Benefits
 

Broad-Spectrum RNase Inhibition: Inhibits common eukaryotic RNases.
Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
Flexible: Functional across wide pH range (pH 5–8) enabling use in many downstream assays.
Recombinant: Made in bacteria, minimizing the chances of human nucleic acid contamination.
Applications
RT-PCR and RT-qPCR.
 

Useful in any applications where eukaryotic RNase contamination is a potential problem.
Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation.
Increase yield and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Performing RNase-free antibody staining.
Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.
 

 

References
 

Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
Lee, F.S. et al. (1989) Biochemistry 28, 225–30.

 

 

注意事項(xiàng):

 

 

QC Tests
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source
Recombinant strain of E. coli (Cat.# N2511, N2515).

Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions
Store at –20°C.

Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.

 

 


? 相關(guān)產(chǎn)品列表:

Promega 系列
序號(hào)貨號(hào)  名稱 規(guī)格
1M1801  T4連接酶         100U 
2M1804      T4連接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         


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