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[供應(yīng)]N2611 RNasin Plus Promega試劑
N2611 RNasin Plus Promega試劑
貨物所在地：
上海上海市
產(chǎn)地：
Promega進口
更新時間：
2025-01-19 21:00:07
有效期：
2025年1月19日--2025年7月19日
已獲點擊：
102
【簡單介紹】N2611 RNasin Plus Promega試劑
規(guī)格：2500u
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【詳細說明】

N2611 RNasin Plus Promega試劑?

原裝*，質(zhì)量保證，現(xiàn)貨供應(yīng)

規(guī)格：2500u

N2611 RNasin Plus Promega試劑? 產(chǎn)品描述：

其它組分：

 

RNasin® Plus RNase Inhibitor【子貨號：N261A，包裝：1 x 2,500u，，運保溫度：–20°C】

 

 

描述：

 

說明

 

RNasin® Plus RNase Inhibitor (RNasin® RNA 酶抑制劑) 是一種作為可溶性蛋白在E. coli 中表達的重組哺乳動物RNase 抑制劑，可以利用離子交換和疏水層析相結(jié)合的方法純化獲得。該蛋白能夠抑制真核RNase( 如RNase A 和RNase B) 活性，與人胎盤RNase 抑制劑相似。RNasin®Plus RNase Inhibitor 經(jīng)過RT-PCR 檢驗，能與AMV、M-MLV 和ImProm-II™ Reverse Transcriptases 或Taq 及Tfl DNA Polymerases 兼容。RNasin® Plus RNase Inhibitor 在TaqMan®檢測中發(fā)現(xiàn)也能和定量、實時RT-PCR 兼容。

與人源的這種蛋白相比，該抑制劑具有更高的抗氧化活性。研究表明來源于人的該蛋白的兩個半胱氨酸對氧化非常敏感，并形成二硫鍵封閉了抑制劑的活性位點。由于天然氨基酸的多樣性，RNasin® Plus RNase Inhibitor 不能形成封閉位點的二硫鍵。此外，還發(fā)現(xiàn)這種重組蛋白具有以前從未認識的特性，即在50℃以上能持續(xù)抑制RNase。將RNasin® Plus RNase Inhibitor 和RNase 的溶液加熱再冷卻，不會造成RNase 活性的恢復(fù)——即使溶液溫度超過RNasin®Plus 蛋白的變性溫度。因此，RNasin® Plus RNase Inhibitor 能夠在加熱前、加熱中及加熱后保護RNA，即使在RT-PCR 中*鏈DNA合成溫度下亦如此。將溶液置于70℃，15 分鐘，沒有觀察到RNase的活性恢復(fù)。

 

特點 

. 增強的抗氧化能力：由于天然氨基酸的多樣性，RNasin® Plus RNase Inhibitor 不能形成人源蛋白在氧化條件下會形成的封閉
活性位點的二硫化物。

• 高純度：RNasin® Plus RNase Inhibitor 是E. coli 表達的可溶蛋白，利用離子交換和疏水相互作用層析相結(jié)合的方法很容易純化獲得。不需要直接親和層析。這種方法可產(chǎn)生純度>90% 的抑制劑蛋白，無 E. coli RNase 殘留。

• 與RT-PCR 系統(tǒng)兼容：RNasin® Plus RNase Inhibitor 已被證明可與Access and Access Quick™ RT-PCR Systems、ImPromII™ Reverse Transcription System 和Reverse Transcription System 兼容。同樣也被證明可與基于TaqMan® 的RT-PCR systems 兼容。

• 在RNA 模板變性過程中保護RNA：即使在70℃加熱RNasin® Plus RNase Inhibitor 和RNase 的混合物15 分鐘，也不會導(dǎo)致RNA 酶的活性復(fù)蘇。許多RT-PCR 操作方法需要在RT反應(yīng)前將RNA 模板在RT 引物存在時變性( 例如65-70℃，5-10 分鐘) 以獲得zui大靈敏度?，F(xiàn)在您可以在這一步驟中加入RNasin® PlusRNase Inhibitor。

• 可保護較高溫度下的RT 反應(yīng)：可在RT 反應(yīng)過程中加入RNasin® Plus RNase Inhibitor，與ImProm-II™ 和AMV Reverse Transcriptases 等一起在50℃以上進行反應(yīng)。在高溫下可能存在的RNase 會失活。

• 可定制或批量訂購：從以下獲得更多關(guān)于該產(chǎn)品的定制選項：www.promega。。ｃｏｍ/myway/。

 

 

 

原廠資料：

 

RNasin® Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor that is expressed as a soluble protein in E. coli, allowing easy purification through a combination of ion exchange and hydrophobic interaction chromatography. The protein is capable of inhibiting eukaryotic RNases (e.g., RNase A and RNase B) similarly to human placental RNase inhibitor. RNasin® Plus RNase Inhibitor is tested in RT-PCR and is compatible with enzymes such as AMV, M-MLV and ImProm-II™ Reverse Transcriptases or Taq and Tfl DNA Polymerases. RNasin® Plus RNase Inhibitor also is tested and compatible with quantitative, real-time RT-PCR in a TaqMan® assay.

The inhibitor offers increased resistance to oxidation over the human version of the protein. Two cysteines in the human protein have been identified as especially sensitive to oxidation and react by forming a disulfide bond that can block the active site of the inhibitor. RNasin® Plus, through natural amino acid diversity, lacks the ability to form this site-blocking disulfide. In addition, the new protein has characteristics never before realized, including continued inhibition of RNases above 50°C. Heating solutions of RNasin® Plus and RNase followed by cooling does not result in the reappearance of RNase activity—even when the solution is heated above the denaturation temperature of the RNasin® Plus protein alone. This allows RNasin® Plus to protect RNA species prior to, during and after heating, even at temperatures normally used during first-strand DNA synthesis in RT-PCR. We have taken solutions up to 70°C for 15 minutes and did not see RNase reactivation.

Features - Benefits

  • Improved Resistance to Oxidation: Due to natural amino acid diversity, RNasin® Plus lacks the capability to form the active site-blocking disulfide bond that can form in the human protein under oxidative conditions.
  • Improved Purification: RNasin® Plus is expressed by E. coli as a soluble protein, allowing easy purification by a combination of ion exchange and hydrophobic interaction chromatography. No direct affinity chromatography required. The new process yields a >90% pure protein with no E. coli RNase carryover.
  • Proven Compatibility with RT-PCR Systems: RNasin® Plus has proven compatible with the Access and AccessQuick™ RT-PCR Systems, ImProm-II™ Reverse Transcription System and the Reverse Transcription System. Also proven compatible with TaqMan®-based RT-PCR Systems.
  • Protection During RNA Template Denaturation: Heating mixtures of RNasin® Plus and RNase does not lead to reactivation of the RNase at temperatures even as high as 70°C for 15 minutes. Many RT-PCR protocols call for RNA template denaturation (e.g., 65–70°C for 5–10 minutes) in the presence of the RT primers prior to full RT reaction assembly for maximum sensitivity. You can now include RNasin® Plus at this step.
  • Protection During Higher Temperature RT Reactions: Add RNasin® Plus during RT reaction assembly and take the reaction to temperatures above 50°C with enzymes like the ImProm-II™ and AMV Reverse Transcriptases. RNases that may be present will not be reactivated at the higher temperature.
  • Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/

Applications

  • RT-PCR.
  • Quantitative, real-time RT-PCR.
  • In vitro transcription.
  • In vitro translation.
  • Coupled transcription/translation.
  • Useful in any applications where eukaryotic RNase contamination is a potential problem.
  • Protection of mRNA in cDNA synthesis reactions.

References

  1. Kim, B.M. et al. (1999) Protein Sci. 8, 430–4.

? 相關(guān)產(chǎn)品列表：

Promega 系列
序號貨號  名稱 規(guī)格
1M1801  T4連接酶         100U 
2M1804      T4連接酶      500U
3M1701      M-MLV              10000U 
4M1705      M-MLV             50000U 
5M5101      AMV                    300U 
6M5108      AMV             1000U  
7M7741      Pfu                      100U
8M7745      Pfu            500U  
9C1101      Olig(dt)15 Primer          20ug 
10M3001      Go-Taq                   100U  
11M3005      Go-Taq                 500U    
12M3008      Go-Taq                2500U     
13U1511      dNTP                   200μl   
14U1515      dNTP                   1000μl  
15A1360      pGEM-T easy              1.2ug   
16A3600      pGEM-T                     1.2ug 
17C1181      Random Primers              20μg  
18N2511      Recombinant RNasin           2500U 
19N2515      Recombinant RNasin      10000U  
20N2611      RNasin Plus                2500U  
21N2615      RNasin Plus              10000U   
22N2111      RNasin                   2500U     
23N2115      RNasin                  10000U   
24M1871      TdT                       300U 
25M1875      TdT                      1500U 
26M2825      CIAP                       1000U  
27M1821      CIAP           1000U            
28M2201      Klenow I      150U        
29M2206      Klenow I        500U           
30M4211      T4 DNA Polymerase  100U      
31M4215      T4 DNA Polymerase   500U          
32M4101      T4 Polynucleotide Kinase 100U          
33M4103      T4 Polynucleotide Kinase  1000U         


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