KDM4D抗體

抗原
Lysine (K)-Specific Demethylase 4D (KDM4D)
別名 JMJD2D, , 4932416A15, Jmjd2d
抗原表位
其他選擇 N-Term
C-Term(12),AA 1-523 (1),AA 61-285 (1)
適用
其他選擇 Human, Monkey
Human(15)
宿主
其他選擇 Rabbit
Rabbit(16)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
APC(1),Alkaline Phosphatase (AP) (1),Biotin (1),FITC (1),HRP (1),PE (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Western Blotting (WB)
Western Blotting (WB)(16),ELISA (7),Immunohistochemistry (IHC) (2),Enzyme Immunoassay (EIA) (1),Immunoassay (IA) (1)
[Pubmed] 2 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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免疫原 Synthetic peptides corresponding to position 357-379 from N-terminal region of Jmj2D peptide sequence
序列 TQK EVQ LPR RAA LGL RQL PSH W
特異性 This antibody detects a single band of approximay 64?kDa of Jmj2D. It does not cross react with other histones or other DNA binding proteins.
交叉反應(yīng) （詳細(xì)） Species reactivity (tested):Human, monkey
純化方法 Affinity chromatography

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 JMJD2D /
背景 Jumonji, AT-rich interactive domain 1C (JARID1C) protein belongs to the highly conserved ARID protein family, which is involved in chromatin remodeling and transcriptional regulation during cell growth, differentiation, and development. In human this gene plays an important role in normal brain development. the bioinformatic prediction indicated several conserved domains incudind an ARID domain, a JmjC domain a C5HC2 zinc finger domain and a JmJ domain. The epigenetic information encoded as methylated CpG di-nucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2. Jumonji domain containing 2D protein (Jmj2D) is a histone demethylase that specifically demethylates'Lys-9' of histone H3, thereby playing a central role in histone code. Jmj2D does not demethylate histone H3 at'Lys-4', H3 at'Lys-27',H3 at 'Lys-36' nor H4 at 'Lys-20'. Jmj2D demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Differential expression of various genes has been studied in esophageal squamous cell carcinomas (ESCs) using genomic hybridization studies. Frequent amplification of DNA copy number has been reported at chromosome 9p23-24 in ESCs that is rich in oncogenes and other tumor-associated genes. One of the novel gene over-expressed in ESCs was cloned and designated as GASC1 or JmjC. The protein contains 2 PD-finger motifs and a P domain. The PHD domains are characteristics of nuclear proteins that participate in chromatin-mediated transcriptional regulation and are present in number of oncogenes (1). Jumonji domain containing protein 2D is a amino acid protein abundantly expressed in brain in gonads suggesting its role in brain and gonads functions. Jumonj3C is a histone demethylase and it demethylates Lys-9 and Lys-36 residues on histone H3 and thus play an important and central role n histone code. Interesting ly, the Jumonji3C does not demethylates histone H3 at position Lys-4, H3 lys-27 nor does it catalyze H4 Lys-20. Jmj3C demethylates trimethylated H3 Lys-9 and Lys-36 residues while it has no activity on mono and dimethylated residues. The enzymatic demethylation of lysine residues generated formaldehyde and succinate. The enzyme also binds to 1?Mole of iron as a cofactor, JmjC domain belongs to the cupin susperfamily that posses protein hydroxylases that catalyze novel histone modifications (2). Jmj3C protein also contains 2 Tudor domains that recognizes and binds methylated histones, the double Tudor domain has in terdigitated structure and an unusual fold that is required for its ability to bind methylated histones by tails.Synonyms: JHDM3D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D, Lysine-specific demethylase 4D
基因ID 55693
NCBI Accession NP_060509
UniProt Q6B0I6

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA: < 1: 10,000. Western blot: > 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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濃度 0.75 - 0.90 mg/mL
緩沖液 Stabilization buffer
注意事項(xiàng) Avoid repeated freezing and thawing.
儲存條件 -20 °C
儲存方法 Store (in aliquots) at -20 °C.

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