PTPRC抗體

抗原
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)
別名 PTPRC, si:dkeyp-47c5.1, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, Cd45, Ly-5, Lyt-4, loc, Lca, RT7, cd45
適用
其他選擇 Chicken
Human(684),Murine (519),Rat ((Rattus)) (142),Cat (56),Bovine (36),Dog (Canine) (32),Chicken (28),Pig (Porcine) (18),Sheep (Ovine) (17),Zebrafish (7),Guinea Pig (5),Primate (4),Monkey (3),Rabbit (3),Goat (2),Non-Human Primate (1),Zebrafish (Brachydanio rerio) (1)
宿主
其他選擇 Mouse
Mouse(700),Rat (492),Rabbit (138),Goat (2),Chicken (1)
克隆類型 (克隆位點)
單克隆 (LT40)
標(biāo)記
其他選擇 FITC
FITC(194),PE (150),Biotin (134),APC (64),Cy5 (21),PerCP (16),PE-Cy7 (13),SPRD (11),Cy5,PE (10),PE,Cy5 (10),PE-Cy5.5 (10),PE-Cy5 (9),Cy7,PE (8),Allophycocyanin (7),PerCP,Cy5.5 (7),APC-Cy7 (6),Alexa Fluor 488 (6),Alexa Fluor 647 (6),HRP (6),DyLight 488 (5),DyLight 550 (5),DyLight 650 (5),APC-Cy5.5 (4),Alexa Fluor 350 (4),Alexa Fluor 555 (4),Alexa Fluor 700 (4),Cy3 (4),Cy5.5 (4),Cy7 (4),EFluor 450 (4),PE,Cy7 (4),APC eFluor 780 (3),DyLight 594 (3),DyLight 680 (3),DyLight 755 (3),PE-TR (3),Alkaline Phosphatase (AP) (2),FLMA (2),Orange Cytognos 515 (2),PE-DyLight 594 (2),PE-Dyomics 590 (2),Texas Red (TR) (2),Cy5.5,PE (1),DY647 (1),Dyomics 647 (1),His tag (1),PE,DY590 (1),PE,DY647 (1),PE,Dyomics 647 (1),PE-Dyomics 647 (1),SureLight® R-PE (1),TRI-COLOR (TC) (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(1070),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (212),Immunoprecipitation (IP) (212),Immunohistochemistry (Frozen Sections) (IHC (fro)) (209),Western Blotting (WB) (196),Immunohistochemistry (IHC) (159),Immunofluorescence (IF) (107),ELISA (74),Immunocytochemistry (ICC) (63),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (55),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (40),Functional Studies (Func) (22),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8),Enzyme Immunoassay (EIA) (6),Cytotoxicity Test (CyTox) (3),Direct Fluorescent Assay (DFA) (2),Immunohistochemistry (Acetone-fixed) (IHC (af)) (2),Indirect Immunofluorescence Assay (IFA) (2),Radioimmunoassay (RIA) (2),Cellular Assay (CA) (1),Microarray (MA) (1)
[Pubmed] 2 references available
規(guī)格 0.5 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 23至28個工作日

PTPRC抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
克隆位點 LT40
亞型 IgM
產(chǎn)品特性 The antibody detects: Chicken CD45 (Mr 190 to 215 kDa).
The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the CD3-CD4-CD8lo immediate precursors to the CD3-CD4+CD8+ thymocytes.

目標(biāo)詳細情況

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目標(biāo)詳細情況

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別名 CD45
背景 Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximay 90 % of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10 % (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3.
研究領(lǐng)域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, Glia marker, CD Antigens, Surface Receptors of Immune Cells

使用細節(jié)

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使用細節(jié)

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應(yīng)用備注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
樣本量 1 mL
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
儲存液 Sodium azide
注意事項 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事項 Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
儲存條件 4 °C

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