CD163 Molecule (CD163) 抗體

抗原
CD163 Molecule (CD163)
別名 DMBT1, CD163, CD163v2, CD163v3, M130, MM130
適用
其他選擇 Rat (Rattus)
Human(139),Rat (Rattus) (29),Pig (Porcine) (18),Murine (17),Bovine (12),Dog (Canine) (12),Monkey (5),Sheep (Ovine) (1)
宿主
其他選擇 Mouse
Mouse(111),Rabbit (54),Chicken (2)
克隆類(lèi)型 (克隆位點(diǎn))
Monoclonal (ED2)
標(biāo)記
其他選擇 PE
Biotin(22),PE (18),FITC (15),APC (4),HRP (3),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Alkaline Phosphatase (AP) (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(83),Western Blotting (WB) (69),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (66),Immunohistochemistry (Frozen Sections) (IHC (fro)) (48),ELISA (30),Immunofluorescence (IF) (20),Immunoprecipitation (IP) (20),Immunocytochemistry (ICC) (14),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunofluorescence (Cultured Cells) (IF (cc)) (9),Immunohistochemistry (IHC) (9),Fluorescence Microscopy (FM) (6),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
[Pubmed] 6 references available
規(guī)格 100 tests
發(fā)貨至 中國(guó) (Change)
發(fā)貨時(shí)間 29至35個(gè)工作日

CD163 Molecule (CD163) 抗體

產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
克隆位點(diǎn) ED2
亞型 IgG1
特異性 This Monoclonal Clone ED2 antibody recognises the Rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Clone ED2 was shown to detect approximay 50?% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiand et al.
無(wú)交叉反應(yīng) Human
交叉反應(yīng) （詳細(xì)） Species reactivity (tested):Rat.
純化方法 Affinity Chromatography on Protein G

目標(biāo)詳細(xì)情況

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CD163 Molecule (CD163) 抗體目標(biāo)詳細(xì)情況

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別名 CD163
背景 CD163 is expressed by approximay 50?% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
基因ID 9332
NCBI Accession NP_004235
UniProt Q86VB7
研究領(lǐng)域 Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 Restore with 1?mL distilled water.
緩沖液 PBS, 0.09?% Sodium Azide, 1?% BSA and 5?% Sucrose
儲(chǔ)存液 Sodium azide
注意事項(xiàng) Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
CD163 Molecule (CD163) 抗體注意事項(xiàng) Avoid repeated freezing and thawing. Dilute only prior to immediate use
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not compley clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.xy-4651R Alpha B Crystallinα晶狀體球蛋白B/αB-crystallin抗體
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