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蛋白質(zhì)提取試劑 TriQuick Reagent
Beijing Solarbio Science & Technology Co., Ltd
Catalog Numbers: R1100 
Quantity :100ml
Storage: 4℃,Keep in Dark Place
Shelflife: 12 months
Product Information:
TriQuick Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA from cell and tissue
samples of animal, plant, yeast, or bacterial origin. It maintains the integrity of the RNA due to highly effective
inhibition of RNase activity while disrupting cells. The RNA can be used in downstream applications such as Northern
blotting, mRNA purification, Invitro-transcription, Rnase-protection assay, RT-PCR and cDNA clonning. TriQuick
Reagent performs well with small quantities of tissue (50-100 mg) and cells (5 × 10 6 ) for 100 times.
Operating Instructions:
Materials Needed( not supplied): Chloroform, Isopropyl alcohol, 75% ethanol, RNase-free water
1. Samples preparation ：
1) Adherent Cells：Remove growth media from culture dish. Add 1 mL TriQuick Reagent directly to the cells in the
culture dish per 10 cm 2  of culture dish surface area. (Note: Add 1 mL TriQuick Reagent for a 35 mm dish, 3 mL for a
60 mm dish, and 8 mL for a 100 mm dish.) Lyse the cells directly in the culture dish by pipetting the cells up and down
several times.
2) Suspension Cells：Harvest cells by centrifugation and remove media. Add 1 mL of TriQuick Reagent to 5× 10 6  –1
×10 7 cells from animal, plant or yeast origin, or 1 × 10 7 cells of bacterial origin. Lyse cells in sample by pipetting up
and down several times. Disruption of some yeast and bacterial cells may require the use of a homogenizer.
3) Tissues：Add 1 mL TriQuick Reagent per 50–100 mg of tissue sample. Homogenize sample using a glass or power
homogenizer. （Note: Process or freeze tissue samples immediay upon collection.）
Incubate the lysing sample for 5 minutes at room temperature to permit complete dissociation of the nucleoprotein
complex . For samples with high content of proteins, polysaccharides, or extracellular material, an additional
centrifugation at 12,000 × g for 10 minutes at 4°C may be required to remove insoluble material from the samples.
Transfer the cleared supernatant to a new tube.
2. Phase separation
Add 0.2 mL of chloroform per 1 mL of TriQuick Reagent used for homogenization. Cap the tube securely. Shake tube
vigorously for 30 seconds. Incubate for 2–3 minutes at room temperature. Centrifuge the sample at 12,000×g for 10
minutes at 4°C. pipetting the aqueous phase into a new tube. Avoid drawing any of the interphase or organic layer into
the pipette when removing the aqueous phase.
3. RNA precipitation
Add 0.5 mL of * isopropanol to the aqueous phase, per 1 mL of TriQuick Reagent used for homogenization.
Incubate at room temperature for 10 minutes. Centrifuge at 12,000 ×g for 10 minutes at 4°C. Remove the
supernatant from the tube, leaving only the RNA pellet. Wash the pellet with 1 mL of 75% ethanol.Vortex the sample
briefly, then centrifuge the tube at 12000×g for 2minutes at 4°C. Discard the wash. Vacuum or air dry the RNA pellet
for 5–10 minutes.
4. RNA resuspension
Resuspend the RNA pellet in RNase-free water (20–50 μL), Proceed to downstream application, or store at –70°C.
Caution
1, The Environment and all equipments used in experiment like tubes, pipets should be Rnase-free to aviod RNA
degradation.
2, Always work with TriQuick Reagent in a fume hood, and always wear a lab coat, gloves and safety glasses. Avoid
direct contact with TriQuick Reagent
Related products ：
R1600  DEPC treated water
P1011  phenol:chloroform: isopropanol ＝ 25 ： 24 ： 1 （ PH<5.0 ）
R1050  5×RNA Loading Buffer
M1010  10×MOPS buffer
SR0020 RNAwait

蛋白質(zhì)提取試劑 TriQuick Reagent訂購(gòu)說明：

1、絕大部分產(chǎn)品備有現(xiàn)貨，一般情況下都能訂貨當(dāng)日發(fā)貨。 
2、部分非常用產(chǎn)品，需提前1－2日預(yù)訂，海外期貨則需要提前3-6周預(yù)訂。 
3、部分產(chǎn)品價(jià)格會(huì)因貨期、批次等因素發(fā)生變化，若有變動(dòng)以訂貨當(dāng)日新價(jià)格為準(zhǔn)。 
4、每日訂單截止時(shí)間為16點(diǎn)整，部分城市可到17點(diǎn)整，因超過截止時(shí)間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
5、請(qǐng)辦理完貨款后，將收據(jù)、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。

蛋白質(zhì)提取試劑 TriQuick Reagent運(yùn)輸說明：

1、極低溫產(chǎn)品：極低溫產(chǎn)品運(yùn)輸過程中加裝干冰運(yùn)輸。用干冰把產(chǎn)品包裹起來，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
2、低溫產(chǎn)品：低溫產(chǎn)品運(yùn)輸過程中加裝索萊寶冰袋運(yùn)輸。事先用冰袋把產(chǎn)品包裹起來，再使用泡沫盒密封，用膠帶嚴(yán)實(shí)密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續(xù)一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
3、常溫產(chǎn)品：常溫產(chǎn)品運(yùn)輸過程中無需加冰或者特殊包裝。產(chǎn)品由公司庫(kù)房人員快速配貨，準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。