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近日，天津醫(yī)科大學(xué)與天津大學(xué)在《Nature Communications 》( IF 14.7 )期刊發(fā)表文獻(xiàn)《Microorganism microneedle micro-engine depth drug delivery》，文獻(xiàn)中的實(shí)驗(yàn)使用了路陽(yáng)儀器上海公司生產(chǎn)的LUYOR-3109臺(tái)式高強(qiáng)度紫外線燈，實(shí)驗(yàn)采用了LUYOR-3109 365nm臺(tái)式大功率紫外線燈用于微針制作過程中的固化。

LUYOR-3109 365nm紫外光源安裝了27w 大功率365nm led，通過二次光學(xué)透鏡進(jìn)行聚焦，紫外光強(qiáng)度超過了傳統(tǒng)400w高壓汞燈的輻照強(qiáng)度，上市以來(lái)，LUYOR-3109已經(jīng)廣泛用于科研院所的科學(xué)實(shí)驗(yàn)室的紫外輻照，目前，使用LUYOR-3109發(fā)表的文獻(xiàn)近百篇。如果您實(shí)驗(yàn)需要一臺(tái)大功率、高強(qiáng)度的365nm紫外光源，路陽(yáng)儀器上海公司提供樣機(jī)免費(fèi)試用。

文獻(xiàn)摘要：

作為一種透皮給藥方法，微針具有微創(chuàng)、無(wú)痛和精確的原位治療。然而，目前的微針依賴于被動(dòng)擴(kuò)散，導(dǎo)致藥物滲透不可控。為了克服這個(gè)問題，我們開發(fā)了一種氣動(dòng)微針貼劑，它使用活的產(chǎn)氣腸桿菌作為微引擎來(lái)主動(dòng)控制藥物輸送。這些微生物產(chǎn)生氣體，將藥物驅(qū)使進(jìn)入更深的組織，可調(diào)節(jié)的葡萄糖濃度可以精確控制該過程。我們的結(jié)果表明，這種微生物驅(qū)動(dòng)的系統(tǒng)將藥物輸送深度增加了 200% 以上，達(dá)到皮膚以下 1000 μm。在銀屑病動(dòng)物模型中，該技術(shù)有效地將骨化三醇輸送到皮下組織，從而快速緩解癥狀。這項(xiàng)創(chuàng)新解決了傳統(tǒng)微針的 局限性，提高了藥物效率、透皮滲透性，并引入了按需受控藥物輸送的創(chuàng)造性范式。

Preparation method of single-layer bacteria-carrying microneedles

First, a solution was prepared by mixing PEGDA and anhydrous ethanol in a 7:3 ratio to make a 10?mL solution. Then, 100?µL of HMPP (1%, v/v) was added to the solution, and it was thoroughly mixed to obtain the substrate solution. Next, a solution of Enterobacter Aerogenes ATCC 13048(E.A.) was placed in a centrifuge tube, and the centrifuge was set to 3000?rpm for 15?min. The resulting centrifuged pellet was thoroughly mixed with the substrate solution to obtain a bacteria-loaded substrate material. Different proportions of glucose solution can be added to the substrate solution as needed to obtain microneedle patches with varying substrate concentrations. Subsequently, a microneedle fabrication mold (microneedle width 200?µm, needle length 500?µm) was taken, and 300?µL of the bacteria-loaded substrate material was added to it. The mold was placed in a vacuum pump for 20?seconds to remove any bubbles from the microneedle mold’s needle array. The mold was then removed, and if necessary, it was exposed to a UV lamp(LUYOR-3109, 27w LED lamp) for 5?s to cure, resulting in complete microneedles. If there was any remaining liquid not drawn into the mold’s pores, an additional 300?µL of the substrate solution was added to fill the mold. A UV lamp was used to cure it for 5?s, and the microneedles were demolded to prepare complete bacteria-loaded microneedles.

文中使用的設(shè)備，另外配置多種不同款式紫外燈，中高強(qiáng)度，強(qiáng)度自由調(diào)節(jié)，物美價(jià)廉