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北京科益恒達(dá)科技有限公司>>液相色譜柱>>德國MN液相色譜柱>> NUCLEOGEL® RP液相色譜柱

NUCLEOGEL® RP液相色譜柱

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更新時間:2018-01-03 15:17:15瀏覽次數(shù):434

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NUCLEOGEL® RP液相色譜柱
Polymer-based reversed phase HPLC phases
for biochemical applications

詳細(xì)介紹

產(chǎn)品特點(diǎn):

NUCLEOGEL® RP

 

Polymer-based reversed phase HPLC phases
for biochemical applications

 

Features

 

 

Polystyrene resin cross-linked with divinylbenzene, available particle sizes 5 μm and 8 μm,
available pore sizes 100 Å and 300 Å ? USP L21
pH working range 113, max. working pressure 180 bar
eluent in column acetonitrile water

Small pore columns for reversed phase separation of small molecules such as pharmaceuticals with basic properties, e.g., organic heterocycles; also suited for separation of nucleosides and nucleotides up to 5000 daltons; allow gradient as well as isocratic elution

Wide pore columns especially recommended for large biomolecules (proteins, peptides etc.),
higher background hydrophobicity compared to silica phases

For more applications click here.


      NUCLEOGEL RP 是苯乙烯-二乙烯苯聚合物為基體的色譜柱系列※      PH應(yīng)用范圍1.0-13.0     100A填料的產(chǎn)品適合堿性藥物的分析※  300A填料的產(chǎn)品適合分子量5000以上的核苷和肽類樣品

產(chǎn)品應(yīng)用:

萬古霉素vancomycin的定量分析

色譜柱:VA 150×4.6mm NUCLEOGEL RP 100-8
流動相:A:8%(v/v) acetonitrile-0.02M borate buffer PH 8.0
       B:16%(v/v) acetonitrile-0.02M borate buffer PH 8.0  梯度洗脫:0-* B in 17.5min
  速:0.5毫升/分鐘
檢測器:UV 235nm

蛋白的分析

色譜柱:VA 50mm×4.6mm  NUCLEOGEL RP 300-8
流動相:A:0.1%TFA 水溶液   梯度洗脫:20-60%   B,22min
  速:1.5毫升/分鐘
檢測器:UV 220nm
  品:1.Ribonuclease  2.Insulin  3.cytochrome c  4.Iysozyme  5.bovine serum albumin6.Myoglobin  7.Ovalbumin

NUCLEOGEL RP C18分析擬交感神經(jīng)胺

色譜柱:VA 150/4.6 NUCLEOGEL 80-10 C18
流動相:A:0.1M 氫氧化胺:乙腈=68:32  B:0.1M氫氧化胺:乙腈=54:46  梯度洗脫0-* B,5min
  速:1.0毫升/分鐘    進(jìn)樣量:20 ul
  度:室溫             檢測器:UV 230nm
  品:1.Phenylpropanolamine   2.Pseudoephedrine   3.Amphetamine   4.Methamphetamine

 

 

訂貨信息:

Ordering information

 

 

Length ->

50 mm

150 mm

250 mm

 

Guard columns*

 

Valco type analytical columns      

NUCLEOGEL® RP 100-5 (particle size 5 µm, pore size 100 Å)

 

4.6 mm ID

 

 

719454

719455

 

719542

 

NUCLEOGEL® RP 100-8 (particle size 8 µm, pore size 100 Å)

 

4.6 mm ID

 

 

719456

719520

 

719542

 

NUCLEOGEL® RP 300-5 (particle size 5 µm, pore size 300 Å)

 

4.6 mm ID

 

719459

 

 

 

719542

 

NUCLEOGEL® RP 300-8 (particle size 8 µm, pore size 300 Å)

 

4.6 mm ID

 

719460

 

 

 

719542

 

* Valco type guard column cartridges measure 5 x 3 mm, require guard column holder B (REF 719539) and are supplied in packs of 2. Columns are supplied in packs of 1.

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