技術(shù)文章
A172 人腦膠質(zhì)瘤細(xì)胞
閱讀:920 發(fā)布時(shí)間:2017-1-17A172 人腦膠質(zhì)瘤細(xì)胞
General Information:
Organism: | Homo sapiens, human |
Tissue: | brain |
Culture Properties: | adherent |
Morphology: | fibroblast |
Culture Method:
Complete Growth Medium: | DMEM (Gibco 12800-017)+10% FBS+ Penicillin/Streptomycin |
Subculturing: | Volumes are given for a 25cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
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Subc*tion Ratio: | A subc*tion ratio of 1:3 to 1:8 is recommended |
Cryopreservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |