CCL-92 3T3- Swiss albino 小鼠胚胎成纖維細胞
ATCC® Number: CCL-92™
Designations: 3T3-Swiss albino
Depositors: H Green
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus （mouse）
Morphology: fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Cellular Products: Lysophosphatidylcholine （lyso-PC） induces AP-1 activity and c-jun N-terminal kinase activity （JNK1） by a protein kinase C-independent pathway [26623]
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Isolation: Isolation date: 1962
Virus Susceptibility: polyomavirus; SV40
Reverse Transcript: negative
Cytogenetic Analysis: This is a hypertriploid mouse cell line. The modal chromosome number was 68 occurring in 30% of cells. The rate of cells with higher ploidies was 2.4%.
Age: embryo
Comments: The 3T3 cell line was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos. [5732]
The cells are contact inhibited.
A confluent monolayer yields 40000 cells/sq cm.
Tested and found negative for ectromelia virus （mousepox）.
The cells should be grown in plastic flasks, they do not grow well on some types of glass surfaces.
A saturation density of approximay 50000 cells per sq cm can be reached.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Never allow culture to become compley confluent. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. For plates （50mm） use an inoculum of 3 X 10 exp5 cells per plate and subculture every 3 days. For 75 sq cm flasks use 4 X 10 exp5 cells per flask and subculture every 3 days.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 18 hrs
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2030
irradiated to be used as feeder cells:ATCC 48-X
References: 5732: Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244
21632: Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596
26261: Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482
26262: Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655
26263: Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605
26623: Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219
32307: Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750
32500: Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator （Tas） binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326
33069: Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936
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