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當(dāng)前位置:> 供求商機(jī)> Lentifect™ 純化與未純化慢病毒顆粒
慢病毒載體是將遺傳物質(zhì)傳遞到大多數(shù)細(xì)胞包括難轉(zhuǎn)染和未分化細(xì)胞中的理想工具。慢病毒顆粒在轉(zhuǎn)入細(xì)胞后將轉(zhuǎn)入基因整合到宿主基因組,造成轉(zhuǎn)入基因的持續(xù)穩(wěn)定表達(dá)。盡管如此,生產(chǎn)、濃縮、滴定得到高滴度、批間重復(fù)性好的慢病毒顆粒需要足夠的實(shí)驗(yàn)經(jīng)驗(yàn),且耗費(fèi)大量的時(shí)間。GeneCopoeia(復(fù)能基因)專業(yè)的科研人員能為您提供高質(zhì)量、高滴度的慢病毒顆粒,以快速高效的方式滿足您科研的需求。
GeneCopoeia(復(fù)能基因)可為您提供已包裝好的純化或未純化慢病毒顆粒,也可提供客戶定制慢病毒包裝服務(wù)。高質(zhì)量的質(zhì)粒DNA、EndoFectin™-Lenti慢病毒轉(zhuǎn)染試劑、TiterBoost™滴度增強(qiáng)劑及豐富的實(shí)驗(yàn)經(jīng)驗(yàn)保證了病毒顆粒的高滴度。
已包裝好的慢病毒顆粒 | ![]() |
已包裝好的純化或未純化慢病毒顆粒主要包含以下方面:
已包裝好的未純化慢病毒顆粒滴度可達(dá)107 copies/ ml,純化的慢病毒顆粒滴度可達(dá)109 copies/ ml。Lentifect™慢病毒產(chǎn)品均經(jīng)過嚴(yán)格的質(zhì)量控制,插入序列均經(jīng)過測(cè)序驗(yàn)證和qPCR滴度驗(yàn)證。
客戶定制慢病毒包裝服務(wù) | ![]() |
GeneCopoeia(復(fù)能基因)為您提供包括ORF cDNA, shRNA, miRNA前體和miRNA抑制劑在內(nèi)的慢病毒包裝服務(wù)。服務(wù)周期根據(jù)服務(wù)內(nèi)容不同而有所差異,zui短為20個(gè)工作日,病毒滴度可達(dá)107 copies/ ml~109 copies/ ml,zui終產(chǎn)品規(guī)格為100 µl 或1 ml。
Panel A. Numbers correspond to the volume of crude eGFP particles used.
Panel B. Numbers correspond to the volume of purified eGFP particles used.
圖1. 未純化的慢病毒顆粒LP-EGFP-LV105 (Panel A)或純化的慢病毒顆粒LPP-EGFP-LV105 (Panel B)與5 µg/ml聚凝胺共感染24孔板中的H1299細(xì)胞。感染72小時(shí)后熒光顯微鏡觀察eGFP表達(dá)。
圖2. 未純化的慢病毒顆粒LP-MCHR-LV105與5 µg/ml聚凝胺共感染24孔板中的H1299細(xì)胞。感染72小時(shí)后熒光顯微鏡觀察eGFP表達(dá)。
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-EGFP-LV105-0200 | eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-EGFP-LV105-0205 | eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-EGFP-LV105-025 | eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
LP-EGFP-LV151-0200 | eGFP Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Neo |
LP-EGFP-LV151-0205 | eGFP Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Neo |
LPP-EGFP-LV151-025 | eGFP Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Neo |
LP-EGFP-LV156-0200 | eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | EF1α | Puro |
LP-EGFP-LV156-0205 | eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | EF1α | Puro |
LPP-EGFP-LV156-025 | eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | EF1α | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-EYFP-LV105-0200 | eYFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-EYFP-LV105-0205 | eYFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-EYFP-LV105-025 | eYFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-ECFP-LV105-0200 | eCFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-ECFP-LV105-0205 | eCFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-ECFP-LV105-025 | eCFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-MCHR-LV105-0200 | mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-MCHR-LV105-0205 | mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-MCHR-LV105-025 | mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-PLUM-LV105-0200 | PLUM Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-PLUM-LV105-0205 | PLUM Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-PLUM-LV105-025 | PLUM Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-FLUC-LV105-0200 | Firefly Luciferase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-FLUC-LV105-0205 | Firefly Luciferase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-FLUC-LV105-025 | Firefly Luciferase Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-RLUC-LV105-0200 | Renilla Luciferase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-RLUC-LV105-0205 | Renilla Luciferase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-RLUC-LV105-025 | Renilla Luciferase Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-MGLUC-LV105-0200 | Gaussia Luciferase Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-MGLUC-LV105-0205 | Gaussia Luciferase Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-MGLUC-LV105-025 | Gaussia Luciferase Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-HLUC-LV201-0200 | Firefly luciferase + eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP/Puro |
LP-HLUC-LV201-0205 | Firefly luciferase + eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP/Puro |
LPP-HLUC-LV201-025 | Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP/Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-HLUC-LV177-0200 | Firefly luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | N/A |
LP-HLUC-LV177-0205 | Firefly luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | N/A |
LPP-HLUC-LV177-025 | Firefly luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | N/A |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-RLUC-LV177-0200 | Renilla luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | N/A |
LP-RLUC-LV177-0205 | Renilla luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | N/A |
LPP-RLUC-LV177-025 | Renilla luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | N/A |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-NEG-LV105-0200 | Negative Control Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-NEG-LV105-0205 | Negative Control Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-NEG-LV105-025 | Negative Control Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
LP-NEG-LV151-0200 | Negative Control Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Neo |
LP-NEG-LV151-0205 | Negative Control Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Neo |
LPP-NEG-LV151-025 | Negative Control Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Neo |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-CMYC-LV105-0200 | c-Myc Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-CMYC-LV105-0205 | c-Myc Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-CMYC-LV105-025 | c-Myc Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-KLF4-LV105-0200 | Klf4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-KLF4-LV105-0205 | Klf4 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-KLF4-LV105-025 | Klf4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-OCT4-LV105-0200 | Oct4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-OCT4-LV105-0205 | Oct4 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-OCT4-LV105-025 | Oct4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-SOX2-LV105-0200 | Sox2 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-SOX2-LV105-0205 | Sox2 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-SOX2-LV105-025 | Sox2 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x108 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-Z8289-LV105-0200 | Nanog Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-Z8289-LV105-0205 | Nanog Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-Z8289-LV105-025 | Nanog Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-LIN28A-LV105-0200 | Lin28 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-LIN28A-LV105-0205 | Lin28 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-LIN28A-LV105-025 | Lin28 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-CRE-LVXM-0200 | CRE recombinase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP Puromycin |
LP-CRE-LVXM-0205 | CRE recombinase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP Puromycin |
LPP-CRE-LVXM-025 | CRE recombinase Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP Puromycin |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-FLP-LVXM-0200 | FLP recombinase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP Puromycin |
LP-FLP-LVXM-0205 | FLP recombinase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP Puromycin |
LPP-FLP-LVXM-025 | FLP recombinase Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP Puromycin |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-SV40T-LV105-0200 | SV40 large T antigen Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-SV40T-LV105-0205 | SV40 large T antigen Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-SV40T-LV105-025 | SV40 large T antigen Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-TAT-LV105-0200 | HIV TAT Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-TAT-LV105-0205 | HIV TAT Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-TAT-LV105-025 | HIV TAT Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
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