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SpeciesReactivity
Mouse

Form
Stds= 0-4000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 125 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified Histone Antigen Coated strip plate ( 8 wells x 12 strips) 5611 2. Mouse Ig?s std A (0 ng/ml), 0.650 ml 5612 3. Mouse Ig?s std B (250 ng/ml), 0.650 ml 5613 4. Mouse Ig?s std C (500 ng/ml), 0.650 ml 5614 5. Mouse Ig?s std D (1000 ng/ml), 0.650 ml 5615 6. Mouse Ig?s std E (2000 ng/ml), 0.650 ml 5616 7. Mouse Ig?s std F (4000 ng/ml), 0.650 ml 5617 8. Goat Anti-mouse IgG HRP conjugate 100X (H+L), 0.12 ml. 5618 9. Sample Diluent (10x), 10 ml SD-10 10. Wash Solution (100X) 10 ml WB-100 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual. M-5610

SpCrossreactivity
Purified histones used in this kit as antigen contains all bands associated with subclasses f1, f2a1, f2a2, f2b, and f3. Anti-histone antibodies, as determined by this kit, will not distinguish between these histone subisotypes. This kit will measure anti-histones toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.