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ROCHE 11814443001- FuGENE® 6 Transfection Reagent *原裝
參考價 | ¥ 3454 |
訂貨量 | ≥1 |
具體成交價以合同協(xié)議為準
- 公司名稱 上海葉舟生物科技有限公司
- 品牌 Roche/羅氏
- 型號 ROCHE 11814443001-
- 產(chǎn)地 ROCHE
- 廠商性質(zhì) 經(jīng)銷商
- 更新時間 2018/2/10 7:41:22
- 訪問次數(shù) 841
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說明書下載:https://www.roche-applied-science.com
產(chǎn)品規(guī)格:1 ml up to 300 transfections
產(chǎn)品貨號:11814443001
產(chǎn)品名稱:FuGENE® 6 Transfection Reagent
產(chǎn)品價格:3454
Application
FuGENE® 6 Transfection Reagent is a non-liposomal multicomponent reagent that is perfect for experiments involving cellular analysis. Its characteristics (listed under "Benefits" below) make it ideal for transfection of a wide range of cells.
Note: To see a list of more than 700 types of cells that have been successfully transfected with FuGENE® 6 Transfection Reagent, including many "hard-to-transfect" cell types, visit our Transfection Special Interest Site and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.
For life science research only.
Benefits
Works on a broad range of cells, including many "hard-to-transfect" cells.
More likely to generate results that are physiologically relevant, since it has very low cytotoxicity and does not disturb cell mechanisms. A recent gene-chip study (Figure 1) demonstrated that transfection reagent from a competitor significantly influenced the expression of more than 2,000 genes, while FuGENE® 6 Transfection Reagent influenced only 30 genes.
Figure 1: Alterations in gene expression following transfection with FuGENE® 6 Transfection Reagent or Reagent L. The number of genes affected after transfection with FuGENE® 6 Transfection Reagent (c) is compared with those affected after transfection with reagent L (d). Changes in the genes expressed by the plasmid without insert (a) and the plasmid coding for secreted human placental alkaline phosphatase (SEAP) (b) were compared after transfection with the two transfection reagents. Overlapping areas represent genes with altered expression levels on all the plasmids (c and d) or with both transfection reagents (a and b).
Allows you to transfect in serum-free or serum-containing media. FuGENE® 6 Transfection Reagent is highly efficient both in the presence and absence of serum (Figure 2). In most cases, the same cell culture medium used for routine cell culture (e.g., one that contains serum) can also be used during transfection. This is especially useful in cases where transfection efficiency improves in the presence of serum.
Important note: The FuGENE® 6 Transfection Reagent and plasmid complex can be added directly to cells that are c*ted in complete medium with FCS and other additives. However, serum-free medium without any additives must be used for dilution of FuGENE® 6 Transfection Reagent and the formation of the complex.
Figure 2: Transfection in the presence or absence of serum. Comparison of transfection efficiency of FuGENE® 6 Transfection Reagent in the presence (10%) or absence of fetal bovine serum. COS-1 cells were transfected with pCMV β-Gal plasmid (2 µg/well) and varying amounts of FuGENE® 6 Transfection Reagent. Cells were lysed and harvested 27 hours post-transfection. 5 µl of lysate was analyzed using the Chemiluminescence β-Gal Reporter Gene Assay.
Saves time and effort by eliminating many handling steps. Procedures that use FuGENE® 6 Transfection Reagent are simple and require minimal optimization (Figure 3).
Figure 3: Comparison of transfection procedures required with FuGENE® 6 Transfection Reagent and another widely used transfection reagent.
Saves money, since you use less reagent per transfection. You can perform more transfections with 1 ml of FuGENE® 6 Transfection Reagent than with 1 ml of reagents from competitor.
Generates a higher yield of stable transfections, since it produces higher cell-survival rates.
Product Description
Composition: contains a proprietary blend of lipids and other components in 80% ethanol.
Sterility: filter-sterilized.
Background Information
Mentioned in over 8,500 publications, FuGENE® 6 Transfection Reagent is one of the most widely used transfection reagents in the world.
Contents
Lipid blend in 80% ethanol, sterile-filtered, packaged in glass vials.
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