胰島素檢測試劑盒

科潤達生物是德國DRG的代理商，提供優(yōu)質(zhì)的胰島素檢測試劑，分別可檢測大鼠胰島素、小鼠胰島素和人胰島素，本文介紹的胰島素INS檢測試劑盒（糖代謝）貨號為EIA2935是一款科研用途試劑盒，定量檢測適用于科研工作，該試劑盒采用ELISA方法進行檢測。該操作方法簡單方便，靈敏度高特異性強的特點，可同時檢測96人份節(jié)省時間成本，酶標(biāo)儀自動讀取數(shù)據(jù)。檢測結(jié)果準確。下面主要介紹三個檢測物種的胰島素檢測試劑的介紹和參考資料。

胰島素介紹

胰島素是由*內(nèi)的胰島β細胞受到內(nèi)源性或外源性的物質(zhì)刺激，如葡萄糖、乳糖、核糖、精氨酸、胰高血糖素等的刺激而分泌的一種蛋白質(zhì)激素。它是人體內(nèi)*的具有降糖作用的激素。胰島素是機體內(nèi)*降低血糖的激素，同時促進糖原、脂肪、蛋白質(zhì)合成。

其主要的生理作用：1、調(diào)節(jié)糖代謝：促進全身組織攝取和利用葡萄糖；2、調(diào)節(jié)脂肪代謝：促進脂肪合成和貯存，減少血液中游離脂肪酸；3、調(diào)節(jié)蛋白質(zhì)代謝：促進氨基酸攝取和蛋白質(zhì)合成，抑制蛋白質(zhì)分解。外源性的胰島素可以用于治療糖尿病。

檢測原理：

DRG公司的這一款胰島素INS檢測試劑盒（糖代謝）檢測方法是酶免法，這一實驗是一種基于夾心法原理的固相酶聯(lián)免吸附實驗(ELISA),反應(yīng)板上的微檢測孔包被有能結(jié)合胰島素分子上特異性的單克隆抗體。一份含有內(nèi)源性胰島素的病人樣本在包被孔中與酶聯(lián)物進行孵育，該酶聯(lián)物是一種生物素標(biāo)記的抗胰島素抗體。孵育后，用洗滌液洗去未結(jié)合的酶聯(lián)物。在第二次溫育時，鏈親和素過氧酶復(fù)合物結(jié)合到生物素化抗胰島素抗體上，過氧酶復(fù)合物的結(jié)合量與樣品中胰島素的濃度成正比。加入底物液后，所顯示的顏色強度與病人樣品中胰島素的濃度成正比

【試劑盒組成成分】

1) 微孔板：12×8，96孔，包被抗胰島素的單克隆抗體。

2) 零標(biāo)準品 ：1支，3ml，即用型，0μIU/ml。

3) 標(biāo)準品1－5：5支，1ml，即用型，6.25;12.5;25;50;100μlU/ml。

4) 酶聯(lián)物：1支，5ml，即用型，生物素化的鼠單克隆抗體。

5) 酶復(fù)合物：1支，7ml，即用型，辣根過氧化物-鏈親和素復(fù)合物。

6) TMB底物液：1支 14ml 即用型。

7) 終止液： 1支 14ml 即用型 包含0.5M硫酸,請勿接觸終止液，以免刺激或著燒皮膚。

8) 洗滌液：1瓶，30ml，40倍濃縮，見試劑準備。

人胰島素檢測試劑盒

小鼠胰島素檢測試劑盒

大鼠胰島素檢測試劑盒

科潤達生物還提供其他糖尿病檢測試劑盒，胰島素樣生長因子-1、C肽、胰島素抗體、IGF-1-600、GIP-1等等，歡迎咨詢我們獲取資料。

Summary and Explanation Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesized in the β-cells of the  islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in  equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain  and B chain (21 and 30 amino acids respectively). The two chains are linked together by two inter-chain disulphide  bridges. There is also an intra-chain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration, and the hormone has a number of important  metabolic actions. Its principal function is to control the uptake and utilisation of glucose in peripheral tissues via the  glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and  glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth  hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus (IDDM) and some other conditions  such as hypopituitarism. Insulin levels are raised in non-insulin-dependent diabetes mellitus (NIDDM), obesity, insulinoma  and some endocrine dysfunctions such as Cushing’s syndrome and acromegaly。

The DRG Insulin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich  principle.  The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on the Insulin  molecule.  An aliquot of patient sample containing endogenous Insulin is incubated in the coated well with enzyme conjugate, which  is an anti-Insulin antibody conjugated with Biotin. After incubation the unbound conjugate is washed off.  During the second incubation step Streptavidin Peroxidase Enzyme Complex binds to the biotin-anti-Insulin antibody. The  amount of bound HRP complex is proportional to the concentration of Insulin in the sample.  Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Insulin in the patient sample.

胰島素INS檢測試劑盒（糖代謝）定量檢測，靈敏度高特異性好，適用于高校科研工作，您可以在此平臺上給我們留言，或者關(guān)注“科潤達生物”公眾號與我們客服聯(lián)系。